Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC150283 | PMC |
http://dx.doi.org/10.1128/JCM.41.3.1252-1255.2003 | DOI Listing |
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