AI Article Synopsis
- The study investigates the significance of pseudouridine (Psi) modifications in rRNA, particularly focusing on their impact on the peptidyl transferase center.
- Depleting certain snoRNAs in yeast led to substantial translation impairment when a conserved Psi in the tRNA A site was lost, while other Psis had minimal effects.
- The findings suggest that some Psis may enhance ribosome function by altering rRNA structure, with potential implications for the role of snoRNPs in modifying ribosome structure.
Article Abstract
One of the oldest questions in RNA science is the role of nucleotide modification. Here, the importance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depleting yeast cells of 1-5 snoRNAs that guide a total of six Psi modifications. Translation was impaired substantially with loss of a conserved Psi in the A site of tRNA binding. Depletion of other Psis had subtle or no apparent effect on activity; however, synergistic effects were observed in some combinations. Pseudouridines are proposed to enhance ribosome activity by altering rRNA folding and interactions, with some Psis having greater effects than others. The possibility that modifying snoRNPs might affect ribosome structure in other ways is also discussed.
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| http://dx.doi.org/10.1016/s1097-2765(03)00040-6 | DOI Listing |
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