AI Article Synopsis
- TILLING (Targeting Induced Local Lesions in Genomes) is a reverse-genetic method that enables the identification of specific point mutations in genes by using chemical mutagenesis.
- High-throughput TILLING allows for the efficient and cost-effective discovery of these mutations across various organisms, as long as sufficient PCR product yield is achieved.
- The Arabidopsis TILLING Project (ATP) has successfully provided over 1,000 mutations in more than 100 genes to researchers within its first year, showcasing the adaptability of TILLING techniques for other species.
Article Abstract
TILLING (Targeting Induced Local Lesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING is dependent only on sufficient yield of PCR products, TILLING can be applied to most organisms. We have developed TILLING as a service to the Arabidopsis community known as the Arabidopsis TILLING Project (ATP). Our goal is to rapidly deliver allelic series of ethylmethanesulfonate-induced mutations in target 1-kb loci requested by the international research community. In the first year of public operation, ATP has discovered, sequenced, and delivered >1000 mutations in >100 genes ordered by Arabidopsis researchers. The tools and methodologies described here can be adapted to create similar facilities for other organisms.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC430291 | PMC |
| http://dx.doi.org/10.1101/gr.977903 | DOI Listing |
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