Determination of proteins with Fast Red VR by a corrected resonance light-scattering technique.

Anal Sci

Institute of Environmental Chemistry, College of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, The People's Republic of China.

Published: February 2003

A simple corrected resonance light-scattering (CRLS) technique was established to correct for any distortion of the resonance light scattering (RLS) spectra resulting from molecular absorption. By using an absorption cell holder to change the propagation direction of the incident light beam of a common spectrofluorometer, the molecular absorption was directly measured through a spectrofluorometer. With measurements of the CRLS signals of the interaction of Fast Red VR (FRV) and proteins, we proved that the present correction for the RLS spectra in terms of the molecular absorption of excitation and scattering radiation can improve the detection sensitivity by about two fold.

Download full-text PDF

Source
http://dx.doi.org/10.2116/analsci.19.211DOI Listing

Publication Analysis

Top Keywords

molecular absorption
12
fast red
8
corrected resonance
8
resonance light-scattering
8
rls spectra
8
determination proteins
4
proteins fast
4
red corrected
4
light-scattering technique
4
technique simple
4

Similar Publications

Methane (CH), which is the main component of natural gas, is an abundant and widely available carbon resource. However, CH has a low energy density of only 36 kJ L under ambient conditions, which is significantly lower than that of gasoline (. 34 MJ L).

View Article and Find Full Text PDF

Rationally manipulating molecular planarity to improve molar absorptivity, NIR-II brightness, and photothermal effect for tumor phototheranostics.

Biomaterials

January 2025

Lab of Molecular Imaging and Translational Medicine (MITM), Engineering Research Center of Molecular and Neuro Imaging, Ministry of Education, School of Life Science and Technology, Xidian University & International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and Treatment, Xi'an, Shaanxi, 710126, China. Electronic address:

The secondary near-infrared region (NIR-II) fluorescence imaging-guided photothermal therapy (PTT) offers a noninvasive and light-controllable treatment option for deep-seated cancers. However, the development of NIR-II photothermal agents (NIR-II PTAs) that possess the desired properties of high molar absorption coefficient (ε), fluorescence quantum yield (QY), and photothermal conversion efficiency (PCE) remain a challenge due to the contradiction between radiative and nonradiative processes. Herein, we propose a novel side-chain heteroatom substitution engineering strategy to simultaneously enhance ε, QY, and PCE by modifying the molecular planarity.

View Article and Find Full Text PDF

The surface termination of a Fe (III) spin crossover molecular salt.

J Phys Condens Matter

January 2025

Department of Physics and Astronomy, University of Nebraska, 855 North 16th Street, Lincoln , 68588-0299, UNITED STATES.

From a comparison of the known molecular stoichiometry and X-ray photoemission spectroscopy (XPS), it is evident that the Fe(III) spin crossover salt [Fe(qsal)2Ni(dmit)2], where qsal = N(8quinolyl)salicylaldimine, and dmit2- = 1,3-dithiol-2-thione-4,5-dithiolato has a preferential surface termination with the Ni(dmit)2 moiety. This preferential surface termination leads to a significant surface to bulk core level shift for the Ni 2p X-ray photoemission core level, not seen in the corresponding Fe 2p core level spectra. A similar surface to bulk core level shift is seen in Pd 3d in the related [Fe(qsal)2]2Pd(dmit)2, ], where qsal = N(8quinolyl)salicylaldimine, and dmit2- = 1,3-dithiol-2-thione-4,5-dithiolato.

View Article and Find Full Text PDF

Vintages for New Fashion: Red-Shifted Photoswitching via the Triplet-Photoreaction Channel with Charge-Transfer Complex Sensitizers.

J Am Chem Soc

January 2025

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, Frontiers Center for Materiobiology and Dynamic Chemistry, East China University of Science and Technology, Shanghai 200237, P. R. China.

Triplet-sensitization has been proven invaluable for creating photoswitches operated over a full visible-light spectrum. While designing efficient triplet-sensitizers is crucial for establishing visible-light photochromism, it remains an appealing yet challenging task. In this work, we propose a versatile strategy to fabricate triplet-sensitizers with intermolecular charge-transfer complexes (CTCs).

View Article and Find Full Text PDF

Absorption-Emission Codes for Atomic and Molecular Quantum Information Platforms.

Phys Rev Lett

December 2024

University of Maryland, NIST, Joint Center for Quantum Information and Computer Science, /, College Park, Maryland 20742, USA.

Diatomic molecular codes [V. V. Albert, J.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!