AI Article Synopsis
- The study aimed to evaluate the immunization effects of different injection routes of a gene vaccine (pcDNA-gtfB) that targets the glucosyltransferase-B enzyme, which is linked to dental cavities in rats.
- Eighteen Wistar rats were split into three groups receiving injections in the quadriceps, intranasally, and in the submandibular gland, with their immune responses measured by levels of serum IgG and salivary IgA.
- Results showed that the quadriceps injection significantly increased serum IgG levels, while the submandibular gland injection notably raised salivary IgA levels, suggesting the gene vaccine is promising for preventing dental caries, especially with targeted immunization.
Article Abstract
Objective: Glucosyltransferase-B (GTF-B) of Streptococcus mutans has been implicated as a principal virulent factor in the development of dental caries. The objective was to use recombined plasmid pcDNA-gtfB expressing multiple antigen of glucosyltransferase-B as gene vaccine to immunize rats through different route, and to investigate the immunization effects of immunization routes.
Methods: A total of 18 Wistar rats were divided into 3 groups, including the quadriceps injection group, the intransal irrigation group and the submandibular gland-targeted injection group. The serum IgG and salivary IgA were assayed by using ELISA after pcDNA3-gtfB immunization. The serum IgG and salivary IgA in different groups were compared using statistical one-way ANOVA.
Results: Compared these 3 groups, the serum IgG in the quadriceps injection group was much higher than those of other two groups (P < 0.01), while the salivary IgA of the submandibular gland-targeted injection was much higher than those of other two groups (P < 0.01).
Conclusion: It is indicated pcDNA3-gtfB is good candidate for anticarious gene vaccine, and submandibular gland-targeted injection is an effective immunization route for stimulating salivary IgA.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Effect of Continuous Intake of WB21 on Tissues Surrounding Implants: A Double-Blind Randomized Clinical Trial.
Life (Basel)
November 2024
Section of Oral Implantology, Department of Oral Rehabilitation, Fukuoka Dental College, 2-15-1 Tamura, Sawara-ku, Fukuoka 814-0193, Japan.
Objective: This study aimed to improve the health of peri-implant tissues through continuous intake of WB21 (LSWB21) tablets.
Methods: A double-blind, randomized controlled trial was conducted with 23 maintenance patients who had generally healthy oral peri-implant tissues. Participants were divided into a test group ( = 12) receiving LSWB21 tablets and a control group ( = 11) receiving placebos.
Salivary Immunoglobulin a Alterations in Health and Disease: A Bibliometric Analysis of Diagnostic Trends from 2009 to 2024.
Antibodies (Basel)
November 2024
Department of Conservative Dentistry and Endodontics, Poznan University of Medical Sciences, 60-812 Poznan, Poland.
Background/objectives: Salivary immunoglobulin A (IgA) is a mediator of local immunity and host defence. Altered IgA levels may predispose to bacterial invasion of the mucosa in the gastrointestinal tract, including the oral cavity. Our study aimed to present the diagnostic trends related to salivary IgA in health and disease based on a bibliometric analysis of published papers between 2009 and 2024.
View Article and Find Full Text PDFExperimental Murine Periodontitis Increases Salivary Gland IgA-Producing B Cells Following Oral Dysbiosis.
Microbiol Immunol
December 2024
Department of Oral Microbiology and Immunology, Showa University Graduate School of Dentistry, Shinagawa-ku, Tokyo, Japan.
Article Synopsis
- The study explores how periodontal inflammation affects both salivary gland function and the oral microbiome, highlighting the significance of these factors in overall health.
- In an experimental model with mice, researchers noticed changes in immune cell activity in the salivary glands and alterations in the oral microbiome following induced periodontitis.
- The findings emphasize a strong connection between oral bacteria, immune responses in salivary glands, and how inflammation can disrupt oral health, providing insights into maintaining oral homeostasis.
Changes in salivary biomarkers of pain, anxiety, stress, and inflammation related to tooth movement during orthodontic treatment: a systematic review.
Dental Press J Orthod
December 2024
São Leopoldo Mandic School of Dentistry, Department of Molecular Biology (Campinas/SP, Brazil).
Objective: This systematic review aimed to analyze the literature on changes in endogenous salivary biomarkers of pain, anxiety, stress, and inflammation related to tooth movement during orthodontic treatment of children and adolescents.
Material And Methods: An electronic search was performed in nine databases to identify quasi-experimental studies, without restricting publication language and year. Two reviewers extracted the data and assessed the individual risk of bias using the JBI tools, and the certainty of evidence using the GRADE tool.
Abatacept use for 24 weeks has a limited effect on salivary gland inflammation in Sjögren's disease patients.
Clin Exp Rheumatol
December 2024
Department of Rheumatology and Clinical Immunology, University of Groningen and University Medical Center Groningen, The Netherlands.
Objectives: This study aimed to assess (1) effects of abatacept on salivary gland histology of Sjögren's disease (SjD) patients, (2) the predictive value of salivary gland histopathological characteristics at baseline for clinical response to abatacept treatment.
Methods: Patients (n=41) who participated in the Dutch ASAP-II and ASAP-III trials and international abatacept trial (IM101603) from whom a labial (n=13) or parotid (n=28) salivary gland biopsy was obtained at baseline and after 24 weeks of treatment with abatacept were included. Biopsies were analysed for SjD related histopathological features before and after abatacept (n=25) or placebo (n=16) treatment.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!