In the five diagnostic tests for H. pylori infection recommended by the Japanese guideline, culture method may be the most difficult in clinical use, because of taking 3 to 5 days for growth, requiring a lot of skill and equipment, and having a possibility of false negative. On the other hand, it has merits of having a specificity of 100%(direct demonstration of the presence of H. pylori), allowing further investigation of the organism (determining its sensitivity to antibiotics, investigating its virulence factors, and typing it for epidemiological purposes). This method is necessary for the cases in whom strains are needed its sensitivity to antibiotics. Considering of increase of CAM-resistant H. pylori in the future, culture method should be necesary in the diagnosis of H. pylori.
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