Crystallization and preliminary crystallographic studies of mung bean cytokinin-specific binding protein.

Acta Crystallogr D Biol Crystallogr

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland, and Institute of Technical Biochemistry, Technical University of Łódź, Poland.

Published: March 2003

Cytokinins, or plant growth hormones, bind with very high affinity to cytokinin-specific binding proteins (CSBPs). Recombinant mung bean CSBP has been overexpressed in Escherichia coli and crystallized in complex with zeatin, a natural plant growth hormone. The crystals belong to the hexagonal system, space group P6(2) or P6(4), with unit-cell parameters a = 113.62, c = 86.85 A, contain two to five copies of the protein in the asymmetric unit and diffract X-rays to 1.25 A resolution.

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http://dx.doi.org/10.1107/s0907444902022916DOI Listing

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