The modeling, synthesis, and characterization of oligomers containing 2-aminoquinazolin-5-yl 2'-deoxynucleotide residues are reported. The 2-aminoquinazoline residues sequence specifically bind via Hoogsteen base pairing as a third strand in the center of the major groove at T:A base pair Watson-Crick duplex sequences. Evidence for the formation of a sequence specific three-stranded structure is based on thermal denaturation UV-vis and fluorescence studies. The novel 2-aminoquinazoline C-nucleotide is a component of a system designed to overcome the homopurine requirement for triple helix structures.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ja028033x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In solid-state nuclear magnetic resonance (ssNMR) spectroscopy, fast magic angle spinning (MAS) is a potent technique that efficiently reduces line broadening and makes it possible to probe structural details of biological systems in high resolution. However, its utilization in studying complex heterogeneous biomaterials such as bone in their native state has been limited. The present study has demonstrated the feasibility of acquiring two-dimensional (2D) H-H correlation spectra for native bone using multiple-quantum/single-quantum correlation experiments (MQ/SQ) at fast MAS (70 kHz).
View Article and Find Full Text PDFDesigning lysyl hydroxylase inhibitors for oral submucous fibrosis - Insights from molecular dynamics.
Int J Biol Macromol
December 2024
Center for Biotechnology, Anna University, Chennai 600 025, India. Electronic address:
Alpha-ketoglutarate (αKG) dependent Lysyl hydroxylase (LH) is a critical enzyme in the post-translational conversion of lysine into hydroxylysine in collagen triple helix and telopeptide regions. Overexpression of LH increases collagen hydroxylation and covalent cross-linkage, causing fibrosis. Currently, no drugs are available to inhibit LH potentially.
View Article and Find Full Text PDFThe Effect of Cellulose Nanocrystals on the Molecular Organization, Thermomechanical, and Shape Memory Properties of Gelatin-Matrix Composite Films.
Gels
November 2024
Biopolymer Research & Engineering Laboratory (BIOPREL), Escuela de Nutrición y Dietética, Facultad de Medicina, Universidad de Los Andes, Santiago 7550000, Chile.
Gelatin is a natural hydrocolloid with excellent film-forming properties, high processability, and tremendous potential in the field of edible coatings and food packaging. However, its reinforcing by materials such as cellulose nanocrystals (CNC) is often necessary to improve its mechanical behavior, including shape memory properties. Since the interaction between these polymers is complex and its mechanism still remains unclear, this work aimed to study the effect of low concentrations of CNC (2, 6, and 10 weight%) on the molecular organization, thermomechanical, and shape memory properties in mammalian gelatin-based composite films at low moisture content (~10 weight% dry base).
View Article and Find Full Text PDFPreparation, characterization and in vitro antioxidant activities of a homogeneous polysaccharide from Prunella vulgaris.
Fitoterapia
December 2024
State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China; Haihe Laboratory of Modern Chinese Medicine, 10 Poyanghu Road, Tianjin 301617, China. Electronic address:
Prunella vulgaris is a medicinal and edible homologous plant, commonly used as a folk medicine to treat diseases. The Prunella vulgaris polysaccharides (PVPs) are reported with the antioxidant activity. This work was designed to isolate, characterize, and test the antioxidant activity of purified PVPs from P.
View Article and Find Full Text PDFEffects of polysaccharide fractions isolated from the pulp of rose laevigata Michx fruit on the foaming and polarization of RAW264.7 macrophage cells.
Int J Biol Macromol
December 2024
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China. Electronic address:
The foaming and polarization of macrophages are pivotal in the formation and development of atherosclerosis. This study delved into the structure and membrane pattern recognition receptors (PRRs) of the neutral polysaccharide fraction (PPRLMF-1), investigating effects of PPRLMF-1 and acid polysaccharide fraction (PPRLMF-2) on the foaming and polarization of RAW264.7 macrophage cells, and exploring their underlying mechanisms.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!