Human epidermal growth factor (hEGF) secreted by recombinant Escherichia coli was purified from culture broth by expanded-bed adsorption (EBA) chromatography, strong anion-exchange chromatography and finally preparative reversed-phase HPLC (RP-HPLC). The EBA chromatography step simultaneously captured the hEGF by cationic exchanger and removed the cellular biomass from the diluted culture broth. This step was carried out at high throughput, and resulted in a high yield (>90%) and a purification factor of approx. 20-fold to >80% purity. Its process performance was well maintained during a 16-fold scale-up. After the successive purification steps of anion-exchange chromatography and RP-HPLC, the overall yield was approx. 84% and the purity was satisfactory (>99.5%). It was concluded that the purification process was very efficient and scaleable, warranting its implementation in large-scale manufacturing.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1042/BA20020113 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Phenotypic and Transcriptomic Changes in Acinetobacter baumannii in Rich and Minimal Growth Mediums.
Can J Microbiol
January 2025
University of Manitoba, Department of Microbiology, Winnipeg, Canada;
Acinetobacter baumannii is an opportunistic pathogen that is often studied in commonly used rich media in laboratories worldwide. Due to the metabolic versatility of A. baumannii, it can be cultured in different growth mediums; however, this can lead to genotypic and phenotypic variations.
View Article and Find Full Text PDFImported seafood is a reservoir of Enterobacteriaceae carrying CTX-M-encoding genes of high clinical relevance.
Int J Food Microbiol
January 2025
Department of Pathobiology and Population Sciences, Royal Veterinary College, NW1 0TU London, United Kingdom; Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark. Electronic address:
We determined the frequency, genotypes, phenotypes, and mobility of extended-spectrum β-lactamase (ESBL)-encoding genes in Enterobacteriaceae isolated from retail seafood products. Overall, 288 samples of fresh shrimps, catfish and seabass imported from Asia were collected from three supermarket chains in the UK (96 each). After enrichment in MacConkey broth supplemented with cefotaxime, total DNA was screened for the presence of CTX-M, SHV and TEM by real-time PCR.
View Article and Find Full Text PDFProlonged incubation time unwarranted for acute periprosthetic joint infections.
J Clin Microbiol
January 2025
Department of Medical Microbiology, Infectious Diseases and Infection Prevention, Maastricht University Medical Centre, Maastricht, the Netherlands.
Unlabelled: Current laboratory protocols for periprosthetic joint infections (PJIs) involve a standard 10- to 14-day incubation period. However, recent evidence indicates considerable variability in the time to diagnosis (TTD) between acute and chronic PJIs. TTD is also influenced by the employed culture media and sample types.
View Article and Find Full Text PDFApplication of spray-dried bacteriocins as cheese biopreservatives.
Int J Food Microbiol
January 2025
Instituto de Investigaciones para la Industria Química, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Salta (INIQUI-CONICET-UNSa), Av. Bolivia 5150, A4408FVY Salta, Argentina; Facultad Ciencias de la Salud, Universidad Nacional de Salta (UNSa), Av. Bolivia 5150, A4408FVY Salta, Argentina. Electronic address:
Listeria monocytogenes can contaminate refrigerated ready-to-eat foods, such as cheeses. Enterocins, with a strong listericidal effect, constitute a natural alternative to control this pathogen in food. To optimize their antimicrobial action in food matrices, bacteriocins can be immobilised in edible coatings through spray drying technology which allows the large-scale production of microcapsules of bioactive molecules.
View Article and Find Full Text PDF[Occurrence of methicillin-resistant Staphylococcus aureus strains at the University Hospital Olomouc].
Klin Mikrobiol Infekc Lek
March 2024
Institute of Microbiology, Faculty of Medicine, Palacky University in Olomouc, Czech Repubic, e-mail:
Objective: This study aimed to evaluate the occurrence of methicillin-resistant Staphylococcus aureus (MRSA) at the University Hospital Olomouc (UHO) over a 10-year period (2013-2022).
Material And Methods: Data was obtained from the ENVIS LIMS laboratory information system (DS Soft, Czech Republic, Olomouc) of the Department of Microbiology, UHO, for the period 1/1/2013-31/12/2022. Standard microbiological procedures using the MALDI-TOF MS system (Biotyper Microflex, Bruker Daltonics) were applied for the identification.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!