A novel enzyme with a specific phenylalanine aminopeptidase activity (ApsC) from Aspergillus niger (CBS 120.49) has been characterized. The derived amino acid sequence is not similar to any previously characterized aminopeptidase sequence but does share similarity with some mammalian acyl-peptide hydrolase sequences. ApsC was found to be most active towards phenylalanine beta-naphthylamide (F-beta NA) and phenylalanine para-nitroanilide (F-pNA), but it also displayed activity towards other amino acids with aromatic side chains coupled to beta NA; other amino acids with non-aromatic side chains coupled to either pNA or beta NA were not hydrolyzed or were poorly hydrolyzed. ApsC was not able to hydrolyze N-acetylalanine-pNA, a substrate for acyl-peptide hydrolases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC143615 | PMC |
| http://dx.doi.org/10.1128/AEM.69.2.1246-1250.2003 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The food enzyme triacylglycerol lipase (triacylglycerol acylhydrolase, EC 3.1.1.
View Article and Find Full Text PDFThe food enzyme glucose oxidase (β-d-glucose: oxygen 1-oxidoreductase, EC 1.1.3.
View Article and Find Full Text PDFScale-Up of Tailor-Made Onsite Enzyme Blend From Cassava Peels for Industrial Bioethanol Production.
ScientificWorldJournal
January 2025
Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Bioethanol production is one of the key alternatives for fossil fuel use due to climate change. The study seeks to upscale tailor-made onsite enzyme blends for the bioconversion of cassava peels to bioethanol in simultaneous saccharification and fermentation (SSF) process using cassava peels-degrading fungi. The starch and cellulose contents of peels were determined.
View Article and Find Full Text PDFInvolvement of the intracellular β-glucosidase BGL1B from Aspergillus niger in the regulation of lignocellulose-degrading enzymes' synthesis.
Biotechnol Biofuels Bioprod
January 2025
College of Life Sciences, Henan Agricultural University, 218 Ping-an Ave., Zhengzhou, 450046, China.
Background: Aspergillus niger is an important lignocellulose-degrading enzyme-producing strain. Multiple regulatory factors regulate the synthesis of lignocellulose-degrading enzymes in A. niger.
View Article and Find Full Text PDFDevelopment of translationally active cell lysates from different filamentous fungi for application in cell-free protein synthesis.
Enzyme Microb Technol
January 2025
Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Universitätsplatz 1, Senftenberg 01968, Germany. Electronic address:
There is an enormous potential for cell-free protein synthesis (CFPS) systems based on filamentous fungi in view of their simple, fast and mostly inexpensive cultivation with high biomass space-time yields and in view of their catalytic capacity. In 12 of the 22 different filamentous fungi examined, in vitro translation of at least one of the two reporter proteins GFP and firefly luciferase was detected. The lysates showing translation of a reporter protein usually were able to synthesize a functional cell-free expressed unspecific peroxygenase (UPO) from the basidiomycete Cyclocybe (Agrocybe) aegerita.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!