AI Article Synopsis
- The exact mechanisms behind how DNA-dependent RNA transcription starts are not fully understood.
- Electrical capacitance measurements showed distinct dielectric changes when the YY1 transcription factor bound to the viral DNA.
- These changes suggest that the formation of the protein-DNA complex alters the electrostatic properties, potentially influencing transcription initiation.
Article Abstract
The detailed mechanism behind the processes of DNA-dependent RNA transcription initiation is largely unknown. When transcription initiation factors bind DNA, a significant change in the electrostatic state of the complex can result. Using electrical capacitance measurements of solutions of the YY1 zinc finger transcription initiation factor and the adeno-associated viral P5 promoter DNA, we observed a specific dielectric change when a protein-DNA complex was formed. We propose that complexation results in electrostatic changes that may trigger the markedly different electrical behavior, and offer a possible explanation for our results.
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| http://dx.doi.org/10.1016/s0301-4622(02)00236-3 | DOI Listing |
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