[Obtaining and construction of a novel gene from Schistosoma japonicum by expressed sequence tags].

Objective: To look for new candidate vaccine molecule and characterize new genes from Schistosoma japonicum.

Methods: New genes of S. japonicum were obtained through expressed sequence tags and analyzed on line. One interest cDNA was cloned into anti-sense eukaryotic expression vector.

Results: An interest cDNA JAYL0230 was obtained. Analysis showed that homology existed between its deduced amino acid sequence and defender against apoptotic death 1 of human and pig, etc. Its anti-sense cDNA was cloned into eukaryotic expression vector pEGFP-N3.

Conclusion: The combination of expressed sequence tags and bioinformatics is an effective way to find novel genes of creature. Construction of anti-sense eukaryotic vector is helpful in studying gene's function.