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Measuring XNA polymerase fidelity in a hydrogel particle format.
Nucleic Acids Res
January 2025
Department of Pharmaceutical Sciences, University of California, Irvine, CA 92697-3958, United States.
Growth in the development of engineered polymerases for synthetic biology has led to renewed interest in assays that can measure the fidelity of polymerases that are capable of synthesizing artificial genetic polymers (XNAs). Conventional approaches require purifying the XNA intermediate of a replication cycle (DNA → XNA → DNA) by denaturing polyacrylamide gel electrophoresis, which is a slow, costly, and inefficient process that requires a large-scale transcription reaction and careful extraction of the XNA strand from the gel slice. In an effort to streamline the assay, we developed a purification-free approach in which the XNA transcription and reverse transcription steps occur inside the matrix of a hydrogel-coated magnetic particle.
View Article and Find Full Text PDFPeptide nucleic acid (PNA) clamps reduce amplification of host chloroplast and mitochondria rRNA gene sequences and increase detected diversity in 16S rRNA gene profiling analysis of oak-associated microbiota.
Environ Microbiome
January 2025
School of Natural Sciences, Bangor University, Bangor, UK.
Background: Acquiring representative bacterial 16S rRNA gene community profiles in plant microbiome studies can be challenging due to the excessive co-amplification of host chloroplast and mitochondrial rRNA gene sequences that reduce counts of plant-associated bacterial sequences. Peptide Nucleic Acid (PNA) clamps prevent this by blocking PCR primer binding or binding within the amplified region of non-target DNA to stop the function of DNA polymerase. Here, we applied a universal chloroplast (p)PNA clamp and a newly designed mitochondria (m)PNA clamp to minimise host chloroplast and mitochondria amplification in 16S rRNA gene amplicon profiles of leaf, bark and root tissue of two oak species (Quercus robur and Q.
View Article and Find Full Text PDFFirst Report of Causing Bacterial Leaf and Panicle Blight of Rice in Louisiana, USA.
Plant Dis
January 2025
LSU AgCenter, Plant Pathology and Crop Physiology, Baton Rouge, Louisiana, United States.
In July 2023, panicle and leaf blight-like symptoms were observed from the rice () variety, PVL03, in research field plots in Louisiana (Rayne, LA 70578, USA; 30.21330⁰ N, 92.37309⁰ W).
View Article and Find Full Text PDFFirst Report of Causing Fruit Rot on Fig in China.
Plant Dis
January 2025
Kashi, Xinjiang, China, China;
Fig (Ficus carica L.) holds economic significance in Atushi, Xinjiang, but as fig cultivation expands, disease prevalence has risen. In July 2024, approximately 22% of harvested fig (cv.
View Article and Find Full Text PDFOccurrence of AG-5 Causing Root Rot on in Northwestern China.
Plant Dis
January 2025
Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu, China;
Astragalus mongholicus is a perennial Chinese medicinal herb in the family Leguminosae widely cultivated in China. In September 2023, A. mongholicus plants in a field in Weiyuan County, Gansu Province, showed symptoms of circular or irregular brown, sunken and necrotic lesions, multiple lesions coalesced, and brown longitudinal cracks in the roots.
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