AI Article Synopsis
- - U2AF(65) is a splicing factor that usually helps attach U2 snRNP to pre-mRNAs at 3' splice sites by binding to specific RNA sequences.
- - Interestingly, U2AF(65) can also aid in bringing U1 snRNP to weak 5' splice sites, particularly when those sites have uridine-rich sequences nearby.
- - The effectiveness of U2AF(65) in recruiting U1 snRNP depends on its arginine- and serine-rich domain, which likely contributes to its ability to facilitate RNA-RNA interactions.
Article Abstract
The splicing factor U2AF(65), U2 small nuclear ribonucleoprotein particle (snRNP) auxillary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to pre-mRNAs. We report that U2AF(65) can also promote the recruitment of U1 snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U1 recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1223361 | PMC |
| http://dx.doi.org/10.1042/BJ20021202 | DOI Listing |
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