The flexibility of DNA is of central importance in biology, medicine, materials science, and mechanical engineering. In this study, we report an unprecedented electrochemical approach for investigating the flexibility of a short (typically 20-base), surface end-tethered single-stranded synthetic DNA oligonucleotide and of its postformed DNA duplex, taking as an example the homopolymer (dT)20 sequence in the regime of very high ionic strength ( approximately 1 M).
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http://dx.doi.org/10.1021/ja028640k | DOI Listing |
Nat Commun
December 2024
Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX, USA.
Programmable and modular systems capable of orthogonal genomic and transcriptomic perturbations are crucial for biological research and treating human genetic diseases. Here, we present the minimal versatile genetic perturbation technology (mvGPT), a flexible toolkit designed for simultaneous and orthogonal gene editing, activation, and repression in human cells. The mvGPT combines an engineered compact prime editor (PE), a fusion activator MS2-p65-HSF1 (MPH), and a drive-and-process multiplex array that produces RNAs tailored to different types of genetic perturbation.
View Article and Find Full Text PDFSoc Sci Med
December 2024
University of Houston, Graduate College of Social Work, 3511 Cullen Blvd, Houston, TX, 77204-4013, USA.
Young adults access mental health services at lower rates than both older and younger age groups despite high levels of need. Mental health service use is known to be influenced by prior experiences with treatment, with episodes of symptoms and treatment producing patterns of service use over time, or what we call a "symptom management career". This qualitative study examined the symptom management careers of 55 young adults (ages 18-25) who were admitted to an inpatient, short term, crisis stabilization unit.
View Article and Find Full Text PDFInt J Sports Physiol Perform
December 2024
School of Sport Science, UiT The Arctic University of Norway, Tromsø, Norway.
Introduction: Women have generally lower body size and lean- to fat-mass ratio, lower maximal anaerobic power due to a lower muscle mass, and fewer fast-twitch fibers, although they can show higher resistance to fatigue or greater metabolic flexibility than men. These factors are well known and explain the sex differences in endurance sports such as distance running (10%-12%). Several of these factors-particularly the differences in body composition and skeletal-muscle characteristics-may directly impact vertical displacement and uphill performance.
View Article and Find Full Text PDFBehav Brain Funct
December 2024
Department of Pharmacology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, 359-8513, Japan.
The large-conductance calcium- and voltage-activated potassium (BK) channels, encoded by the KCNMA1 gene, play important roles in neuronal function. Mutations in KCNMA1 have been found in patients with various neurodevelopmental features, including intellectual disability, autism spectrum disorder (ASD), or attention deficit hyperactivity disorder (ADHD). Previous studies of KCNMA1 knockout mice have suggested altered activity patterns and behavioral flexibility, but it remained unclear whether these changes primarily affect immediate behavioral adaptation or longer-term learning processes.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
Department of Plastic and Cosmetic Surgery, Treatment Center of Burn and Trauma, Affiliated Hospital of Jiangnan University, Wuxi 214122, China. Electronic address:
The conversion of mechanical energy into electrical energy by triboelectric nanogenerators (TENG) has attracted attention in recent years, particularly in the field of wearable sensor. In this work, TEMPO-oxidized cellulose nanofibers (TOCNF) with carboxylate groups were compounded with MXene to serve as both the negative friction layer and the electrode in assembling a TENG with nylon. The synergistic effect between TOCNF and MXene was analyzed to disclose its influence on the performance of the as-prepared TENG.
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