A novel gene containing PDZ and LIM domains, PCD1, is overexpressed in human colorectal cancer.

Background: The process of colorectal cancer development involves accumulated genetic alterations affecting APC, K-ras and p53. A recently identified gene, PCD1, was reported to be up-regulated in human malignancies including colorectal cancers, but relationships between PCD1 gene expression and clinicopathological findings, as well as the timing of genetic alteration of PCD1 in colorectal cancer development, are not clear. To determine whether PCD1 contributes to colorectal cancer progression, we investigated the expression of PCD1 mRNA in human colorectal tissues.

Materials And Methods: The expression of PCD1 mRNA was determined by quantitative RT-PCR. The mutation of p53 was detected by a PCR-SSCP method.

Results: Up-regulation of PCD1 gene transcription was observed not in adenomas but in cancers compared to normal mucosa (p < 0.0001). Primary tumors with a mutation of p53 showed significantly greater PCD1 gene expression than tumors without such a mutation (p = 0.0134).

Conclusion: The PCD1 gene may play a role in colorectal cancer development from adenomas.