Circulating human antimouse antibodies (HAMAs) directed to mouse immunoglobulin G (IgG) are clinically significant, compromising mouse antibody therapy and imaging, and interfering in immunological assays. To investigate the HAMA response, 20 stable cell lines secreting human monoclonal antibodies reactive with mouse IgG were established from a donor with a history of exposure to mice. Their subclass and domain specificities were established by solid-phase binding, indirect haemagglutination assays and immunoblotting, using Igs of known subclass and Ig fragments. The heavy-chain variable region gene usage was determined for 12 HAMAs. Eight HAMAs were IgM, 11 HAMAs were IgG4 and one HAMA was IgG1, indicating an IgG4-dominated response. All of the IgG HAMAs reacted with epitopes present on the Fc portion; one was subclass-specific, nine were subclass-restricted and two were pan-IgG-reactive. Measurement of their affinities gave dissociation constants typically in the nanomolar range. Seven and five HAMAs were derived from variable heavy-chain 3 (VH3) and VH1 gene segments, respectively. The IgG HAMAs used different VH segments to the IgM HAMAs. JH regions were coded by JH4 in eight HAMAs. DH segment usage appeared to be restricted in the IgM HAMAs. Two IgG HAMAs were clonally related. These monoclonal HAMAs are potentially useful as reagents for detecting mouse IgG and as reference reagents for the investigation of the HAMA response in patients undergoing mouse monoclonal antibody therapy and for the investigation of the influence of HAMAs on immunodiagnostic tests.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1046/j.1365-3083.2003.01189.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Multidimensional vulnerability and financial risk protection in health in contexts of protracted conflict: Evidence from the Occupied Palestinian Territory.
PLoS One
January 2025
Centre for Health Economics, University of York, Yorkshire, United Kingdom.
This paper proposes a multidimensional vulnerability index for a setting of protracted conflict, which is applied to study the relationship between financial vulnerability and catastrophic healthcare expenditure (CHE) incidence in the Occupied Palestinian Territory in 2018. We find that our index better captures the extent of financial risk protection in health compared to conventional measures of financial welfare. Results indicate that the most vulnerable groups experience a significantly higher likelihood of incurring CHE, and this likelihood is increased for those living in the West Bank compared to the Gaza Strip.
View Article and Find Full Text PDFNonlinear relationship between physical environment risks, investor attentions, and financial systemic risks: Evidence from mLSTM-CoVaR networks.
J Environ Manage
January 2025
School of Management, Lanzhou University, Gansu, China. Electronic address:
How do physical environment risks affect financial market systemic risk? We use remoting data to measure physical environment risks and select 26 banks across 12 EU countries. We extend the CoVaR framework with the quantile-mLSTM algorithm, obtaining time-varying CoVaRs. We then use time-varying partial derivatives to calculate the banks' tail risk spillover effects.
View Article and Find Full Text PDFAntisemitism on Campus in the Wake of October 7: Examining Stress, Coping, and Depressive Symptoms Among Jewish Students.
Stress Health
February 2025
Department of Psychology, The University of British Columbia, Vancouver, Canada.
The Hamas-led terrorist attacks in Israel on October 7, 2023, were an inflection point that spurred a global rise in antisemitism. College and university campuses were particularly affected. Given the adverse impacts of prejudice and discrimination for mental health and the dearth of research on psychosocial effects of antisemitism, examining stress, coping, and mental health among Jewish students within this context is crucial.
View Article and Find Full Text PDFBackground: Mass disasters, whether natural or human-made, pose significant public health challenges, with some individuals demonstrating resilience, whereas others experience persistent emotional distress that may meet diagnostic criteria for mental health disorders. We explored key risk factors for distress following the October 7, 2023, Hamas attacks on Israel, focusing on trauma exposure, gender, and event centrality.
Method: A longitudinal study design was used, assessing posttraumatic distress (PTSD), depression, generalized anxiety, event centrality, and functioning at approximately three (T1; n=858) and seven (T2, n=509) months post-attack.
Trauma echoes: factors associated with peritraumatic distress and anxiety five days following Iranian missile attack on Israel.
Eur J Psychotraumatol
December 2025
Department of Gerontology, University of Haifa, Haifa, Israel.
On 13-14 April 2024, Iran launched ∼300 drones and missiles at Israel, in an unprecedented attack. As most studies examine the effects of trauma months or years later, less is known about its effects days later. To fill this gap, this study gauged the population response, five days after the attack.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!