[reaction: see text] Osmium tetroxide has been microencapsulated in a polyurea matrix using an in situ interfacial polymerization approach. These microcapsules have been effectively used as recoverable and reusable catalysts in the dihydroxylation of olefins
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http://dx.doi.org/10.1021/ol020225+ | DOI Listing |
Toxicol Pathol
January 2025
Premier Laboratory, LLC, Longmont, Colorado, USA.
Hematoxylin and eosin (H&E) staining is a suitable approach for detecting substantial structural changes in neural tissues but is less sensitive for identifying subtle alterations to subcellular structures and various chemical constituents, including myelin. Neurohistological methods to better evaluate myelin integrity by light microscopy include acidophilic dyes (eg, eriochrome cyanine R, toluidine blue [used with hard plastic sections]); lipoprotein-binding dyes (eg, Luxol fast blue [LFB], Weil's iron hematoxylin); lipid impregnation with metals (eg, Marchi's, which uses osmium tetroxide for en bloc staining before embedding); and immunohistochemical (IHC) methods to highlight various antigens (eg, myelin basic protein [MBP] and peripheral myelin protein 22 [PMP22]). Some IHC methods reveal enhanced marker expression in damaged myelin (eg, matrix metalloproteinase-9 [MMP9], S100).
View Article and Find Full Text PDFBioorg Chem
January 2025
Sakarya University, Faculty of Arts and Sciences, Chemistry Department, 54050, Sakarya, Turkey. Electronic address:
In this study, Diels-Alder reaction was performed to sulfolene and endo/exo-diacetate compounds. After a series of reactions, new conduritol A and F analogs containing oxo-bridge and naphthalene rings in their structures were synthesized. To the starting compound, bromination, elimination, singlet oxygen reaction, acetylation, selective oxidation with osmium tetroxide (OsO), and m-chloroperbenzoic acid (m-CPBA), re-acetylation, and finally hydrolysis of the compounds by NH(g)/MeOH reactions were carried out.
View Article and Find Full Text PDFForensic Sci Int
December 2024
Department of Legal Medicine, Faculty of Medicine, University of Toyama, Toyama, Japan.
This study investigated the usefulness of histopathological examination in diagnosing ketoacidosis in hypothermia cases. Out of 79 cases (38 females), 30 showed basal vacuolization (BV), a representative histopathological finding of ketoacidosis, in their hematoxylin and eosin (H&E)-stained specimens. Through the use of both lipid staining with osmium tetroxide postfixation and immunohistochemistry for adipophilin (ADFP-IHC), BV pattern pathology was identified in 43 cases.
View Article and Find Full Text PDFMethodsX
December 2024
Institute of Engineering, Laboratory of Advanced Electron Microscopy, Autonomous University of Baja California, Benito Juárez Blvd. and Normal Street, Mexicali, 21040, Baja California, Mexico.
This method describes the preparation of specimen for scanning electron microscopy (SEM), starting from specimen collection to obtaining SEM images. Our approach involves preserving cellular structure and preventing cell degradation through chemical fixation and post-fixation without Osmium tetroxide (OsO), followed by air dried specimen placed onto Si substrate. Additionally, the method outlines the steps for mounting the prepared specimen onto SEM sample holder aluminum stubs, followed by coating with an ultrathin gold layer via thermal evaporation to enhance specimen/substrate/stub conductivity.
View Article and Find Full Text PDFJ Neuroimaging
November 2024
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA.
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