Recent plans announced for the systematic cataloging of the minimal Escherichia coli gene set, the phenotypes of all mutations, the expression levels of every transcript and gene product, and the interactions of all genetic loci or their gene products point the way towards a new frontier in the biology of model organisms. Powerful tools for this endeavor are emerging, and efforts to organize the E. coli community are under way. The anticipated benefit is a functional model of the bacterial cell.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0966-842x(02)00008-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Engineering a cpGFP-based biosensor for enhanced quantification of glycolate production in Escherichia coli.
Talanta
January 2025
Frontiers Science Center for Synthetic Biology (Ministry of Education), Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China; Haihe Laboratory of Sustainable Chemical Transformations, Tianjin, 300192, China. Electronic address:
The growing demand for glycolate, fueled by economic development, requires the advancement of production methods. Escherichia coli (E. coli), a preferred host for glycolate production, has undergone extensive metabolic engineering to improve yield.
View Article and Find Full Text PDFSimple and Fail-safe Method to Transform Miniprep Strain K12 Plasmid DNA Into Viable EHA105 Cells for Plant Genetic Transformation.
Bio Protoc
January 2025
Department of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya.
Agrobacterium-mediated gene transformation method is a vital molecular biology technique employed to develop transgenic plants. Plants are genetically engineered to develop disease-free varieties, knock out unsettling traits for crop improvement, or incorporate an antigenic protein to make the plant a green factory for edible vaccines. The method's robustness was validated through successful transformations, demonstrating its effectiveness as a standard approach for researchers working in plant biotechnology.
View Article and Find Full Text PDFAn efficient electrocatalytic in-situ hydrogen peroxide generation for ballast water treatment with oxygen groups.
Sci Total Environ
January 2025
Ability R&D Energy Research Centre, School of Energy and Environment, City University of Hong Kong, Hong Kong; State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong.
The in-situ electrochemical production of hydrogen peroxide (HO) offers a promising approach for ballast water treatment. However, further advancements are required to develop electrocatalysts capable of achieving efficient HO generation in seawater environments. Herein, we synthesized two-dimensional lamellated porous carbon nanosheets enriched with oxygen functional groups, which exhibited exceptional performance in HO electrosynthesis.
View Article and Find Full Text PDFChemical Profile and Antibacterial Activity of L. cv Graciano Pomace Extracts Obtained by Green Supercritical CO Extraction Method Against Multidrug-Resistant Strains.
Foods
December 2024
Instituto de Ciencias de la Vid y del Vino (ICVV) (Universidad de La Rioja, Consejo Superior de Investigaciones Científicas (CSIC), Gobierno de La Rioja), 26007 Logroño, Spain.
The objectives of this study were to obtain and characterise polyphenolic extracts from red grape pomace of L. cv Graciano via conventional solvent extraction (SE) and green supercritical fluid extraction (SFE) and to evaluate their antibacterial activity against susceptible and multidrug-resistant strains of intestinal origin. The SE and SFE methods were optimised, and ultra-performance liquid chromatography/mass spectrometry (UPLC/QqQ-MS/MS) analysis revealed 38 phenolic compounds in the SE sample, with anthocyanins being the predominant polyphenols, and 21 phenolic compounds in the SFE samples, among which hydroxybenzoic acids and flavonols were the predominant compounds.
View Article and Find Full Text PDFAntimicrobial Properties of Monomeric and Dimeric Catanionic Surfactant System.
Molecules
January 2025
Department of Bioactive Products, Faculty of Chemistry, Adam Mickiewicz University Poznan, 61-614 Poznan, Poland.
Cationic gemini surfactants are used due to their broad spectrum of activity, especially surface, anticorrosive and antimicrobial properties. Mixtures of cationic and anionic surfactants are also increasingly described. In order to investigate the effect of anionic additive on antimicrobial activity, experimental studies were carried out to obtain MIC (minimal inhibitory concentration) against and bacteria.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!