Background: Superficial mucosal injury is repaired immediately by a process known as restitution, which is controlled by several factors and is based on cellular migration. Heat-shock preconditioning increases the tolerance of tissue to deep gastric mucosal injury via synthesis of heat-shock proteins. Despite immediate positive effects on the tissue, we have shown that heat-shock preconditioning inhibits restitution of gastric mucosa after subsequent superficial injury in correlation with Hsp70 levels. In addition to heat-shock proteins, heat preconditioning is known to affect eicosanoid pathways. In this study, we explore the role of eicosanoid pathways and protein synthesis in heat-shock-induced inhibition of restitution.
Methods: Guinea pig gastric mucosa was mounted and perfused in a Ussing chamber (37 degrees C). After heat-shock preconditioning (HS) (42 degrees C) and normothermic recovery, a superficial injury was induced (1.25 mol/L NaCl) followed by a 3-h restitution. Transmucosal electrophysiologic resistance of the tissue (R) was recorded during the experiment. During perfusion, the mucosa was exposed to 30 micromol/L arachidonic acid as a substrate for eicosanoid pathways; 50 micromol/L quercetin to inhibit the metabolism of arachidonic acid via lipoxygenases, 50 micromol/L indomethacin to inhibit the metabolism of arachidonic acid via cyclo-oxygenases, or 150 micromol/L cycloheximide to inhibit de novo protein synthesis. After the experiment, the mucosa was prepared for morphologic analysis and Western blotting.
Results: HS inhibited restitution after NaCl insult and upregulated Hsp70. Exposure of the tissue to quercetin, cycloheximide, arachidonic acid, or to indomethacin overcame the inhibitory effect of HS, which could be pronounced by simultaneous indomethacin and quercetin augmenting expression of Hsp70.
Conclusion: HS preconditioning inhibits restitution by a mechanism that involves arachidonic acid metabolism and de novo protein synthesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/003655202762671224 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
msiFlow: automated workflows for reproducible and scalable multimodal mass spectrometry imaging and microscopy data analysis.
Nat Commun
January 2025
Institute for Experimental Immunology and Imaging, University Hospital Essen, Essen, Germany.
Multimodal imaging by matrix-assisted laser desorption ionisation mass spectrometry imaging (MALDI MSI) and microscopy holds potential for understanding pathological mechanisms by mapping molecular signatures from the tissue microenvironment to specific cell populations. However, existing software solutions for MALDI MSI data analysis are incomplete, require programming skills and contain laborious manual steps, hindering broadly applicable, reproducible, and high-throughput analysis to generate impactful biological discoveries. Here, we present msiFlow, an accessible open-source, platform-independent and vendor-neutral software for end-to-end, high-throughput, transparent and reproducible analysis of multimodal imaging data.
View Article and Find Full Text PDFThe association between arachidonic acid and gallstone risk: cross-sectional study and Mendelian randomization analysis.
Postgrad Med J
January 2025
Shandong University of Traditional Chinese Medicine Affiliated Hospital, No. 42 Wenhua West Road, Lixia District, Jinan, Shandong Province 250011, China.
Background: The formation of gallstones is a multifactorial process involving lifestyle habits, lipid metabolism disorders, and genetic factors. This study aims to explore the association between 19 types of dietary fatty acids and gallstone disease using large-scale population data, assess the correlation between dietary fatty acids and serum fatty acids, and investigate the causal relationship between plasma lipids and gallstone disease from a genetic perspective.
Methods: We employed a cross-sectional study design, combined with logistic regression analysis to evaluate the association between dietary fatty acids and gallstone disease.
Polysaccharide Modulates Characteristic Bacteria and Metabolites, Improving the Immune Function of Healthy Mice.
Nutrients
January 2025
State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China.
Objectives: Polysaccharides from are known to have several bioactive effects. Previous studies have found that low-molecular-weight polysaccharide (GP1) is degraded by and promotes the production of beneficial bacteria and metabolites, which improves immune disorder and intestinal injury, and then enhances the body's immune regulation ability. However, the immune regulation effect of GP1 on a healthy body has not been studied.
View Article and Find Full Text PDFEtodolac Single Dose Metabolic Profile Elucidation: Pharmacokinetics and Adverse Events in Healthy Volunteers.
Pharmaceuticals (Basel)
January 2025
Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista 12916-900, SP, Brazil.
Background/objectives: This study investigates the metabolic profile of a single dose of etodolac in healthy volunteers, focusing on pharmacokinetics, clinical parameters, and metabolomic variations to identify biomarkers and pathways linked to drug response, efficacy, and safety.
Methods: Thirty-seven healthy volunteers, enrolled after rigorous health assessments, received a single dose of etodolac (Flancox 500 mg). Pharmacokinetic profiles were determined using tandem mass spectrometry analysis, and the metabolomic profiling was conducted using baseline samples (pre-dose) and samples at maximum drug concentration (post-dose) via liquid chromatography coupled with a quadrupole time-of-flight mass spectrometer.
Toxicology Effects of Cadmium in : Accumulation, Oxidative Stress, Microbial Community, and Transcriptome Analysis.
Int J Mol Sci
January 2025
Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Provincial Key Laboratory of Agricultural & Rural Pollution Abatement and Environmental Safety, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Cadmium (Cd) pollution poses an important problem, but limited information is available about the toxicology effects of Cd on freshwater invertebrates. We investigated the accumulation, oxidative stress, microbial community changes, and transcriptomic alterations in apple snails ) under Cd stress. The snails were exposed to the 10 μg/L Cd solution for 16 days, followed by a 16-day elimination period.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!