[Several monomes from Tripterygium wilfordii inhibit proliferation of glioma cells in vitro].

Background And Objective: Researches indicated that Tripterygium wilfordii possess antitumor activity. The current study was designed to investigate inhibitive effect of several monomes of Tripterygium wilfordii on the proliferation of glioma cells.

Methods: The effect of three monomes from Tripterygium wilfordii on the proliferation of glioma cell lines SHG44, C6, and U251 in vitro was examined by using MTT assay. Immunohistochemistry was used to examine the expression of Bax, Bcl-2 after treatment of triptolide and celastrol.

Result: The proliferation of glioma cells was remarkably inhibited by triptolide and celastrol. They both increased expression of Bax and decreased expression of Bcl-2 in the SHG44 cells.

Conclusion: Triptolide and celastrol inhibit the proliferation of glioma cells in vitro, which was associated with promoting the expression of Bax and inhibiting the expression of Bcl-2 and accelerating cell apotosis.