The mechanisms of cochlear hair cell death following exposure to transient inner ear ischemia were investigated in gerbils histologically. The animals were subjected to ischemic insult by occluding both vertebral arteries for 15 min. Hoechst 33342 nuclear staining showed that inner hair cells (IHCs) underwent sporadic degeneration via nuclear condensation, which peaked 12 hours after the ischemia. Furthermore, nuclear DNA fragmentation was noted by the terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling method. Transmission electron microscopy revealed morphological changes in the IHCs characteristic of apoptosis, including karyopyknosis, chromatin condensation. These findings suggest that apoptotic cell death is the major process in hair cell degeneration in this animal model.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00001756-200212200-00017 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Defects in hair cells disrupt the development of auditory peripheral circuitry.
Nat Commun
December 2024
The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA.
Deafness is the most common form of sensory impairment in humans and frequently caused by defects in hair cells of the inner ear. Here we demonstrate that in male mice which model recessive non-syndromic deafness (DFNB6), inactivation of Tmie in hair cells disrupts gene expression in the neurons that innervate them. This includes genes regulating axonal pathfinding and synaptogenesis, two processes that are disrupted in the inner ear of the mutant mice.
View Article and Find Full Text PDFHypoxic Preconditioning Prevents Oxidative Stress-Induced Cell Death in Human Hair Follicle Stem Cells.
Iran J Biotechnol
July 2024
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Objectives: This study investigated the impact of hypoxic preconditioning on the survival and oxidative stress tolerance of nestin-expressing hair follicle stem cells (hHFSCs) and SH-SY5Y neuroblastoma cells, two crucial cell types for central nervous system therapies. The study also examined the relative expression of three key genes, HIF1α, BDNF, and VEGF following hypoxic preconditioning.
Materials And Methods: hHFSCs were isolated from human hair follicles, characterized, and subjected to hypoxia for up to 72 hours.
Clinical differentiation of cutaneous and subcutaneous mast cell tumors in dogs: A pilot study.
Open Vet J
November 2024
Division of Animal Medical Research, Hassen-kai, 2-27 Onozaki, Saito, Miyazaki 881-0012, Japan.
Background: Canine mast cell tumors (MCT) in the skin are classified into cutaneous MCT (cMCT) and subcutaneous MCT (scMCT) types, which exhibit different clinical behaviors. Although these types have been classified only by histology, preoperative differentiation is important for proper surgical planning.
Aim: To examine the accuracy of differentiating these types based on the gross features before surgery.
High-Throughput Screening of 3-Dimensional Co-culture Hair Follicle Mimetic Tissue with an Enhanced Extracellular Matrix for the Screening of Hair Growth-Promoting Compounds.
Biomater Res
December 2024
Department of Molecular Science and Technology, Ajou University, Suwon 16499, South Korea.
Hair follicle cells reside within a complex extracellular matrix (ECM) environment in vivo, where physical and chemical cues regulate their behavior. The ECM is crucial for hair follicle development and regeneration, particularly through epithelial-mesenchymal interactions. Current in vitro models often fail to replicate this complexity, leading to inconsistencies in evaluating hair loss treatments.
View Article and Find Full Text PDFResveratrol-Loaded Versatile Nanovesicle for Alopecia Therapy via Comprehensive Strategies.
Int J Nanomedicine
December 2024
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong Province, People's Republic of China.
Introduction: Alopecia is a systemic disease with multiple contributing factors. Effective treatment is challenging when only hair growth mechanisms are targeted while ignoring the role of maintaining hair follicle microenvironment homeostasis, which is crucial for cell growth and angiogenesis. Oxidative stress and inflammation are major disruptors of this microenvironment, leading to inhibited cell proliferation and compromised hair follicle circulation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!