AI Article Synopsis
- Id proteins are transcription factors that play a role in regulating tumor blood vessel formation.
- Through experiments with normal and Id1-deficient mouse cells, researchers found that Id1 represses thrombospondin-1 (TSP-1), which inhibits angiogenesis.
- Studies showed that Id1 knockout mice had less new blood vessel formation, which could be reversed by targeting TSP-1, highlighting its importance in the process.
Article Abstract
Id proteins are helix-loop-helix transcription factors that regulate tumor angiogenesis. In order to identify downstream effectors of Id1 involved in the regulation of angiogenesis, we performed PCR-select subtractive hybridization on wild-type and Id1 knockout mouse embryo fibroblasts (MEFs). Here we demonstrate that thrombospondin-1 (TSP-1), a potent inhibitor of angiogenesis, is a target of transcriptional repression by Id1. We also show that Id1-null MEFs secrete an inhibitor of endothelial cell migration, which is completely inactivated by depletion of TSP-1. Furthermore, in vivo studies revealed decreased neovascularization in matrigel assays in Id1-null mice compared to their wild-type littermates. This decrease was completely reversed by a TSP-1 neutralizing antibody. We conclude that TSP-1 is a major target for Id1 effects on angiogenesis.
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| http://dx.doi.org/10.1016/s1535-6108(02)00209-x | DOI Listing |
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