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Choice of different dyes to label tyrosine and nitrotyrosine. | LitMetric

Choice of different dyes to label tyrosine and nitrotyrosine.

J Chromatogr A

Laboratoire de Biochimie CHR Rangueil, 31043 Toulouse Cedex, France.

Published: December 2002

In this work, we will present some attempts to analyze tyrosine and nitrotyrosine using capillary electrophoresis and either UV-Visible detection or laser-induced fluorescence (LIF) detection. An argon ion (488 nm) laser is used for fluorescein isothiocyanate (FITC) and 7-fluoro-4-nitro-2,1,3-benzoxadiazole (NBD-F). A near infrared (780 nm) laser is used for NIR 780 derivatives. The UV-Visible limit of detection is 2.5 microM whereas it is in the range of 30 nM for LIF detection.

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http://dx.doi.org/10.1016/s0021-9673(02)01502-9DOI Listing

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