Background: Recent experimental and clinical studies have shown that cardiomyopathy (CMP) can be associated with disorders of oxidative metabolism in cardiomyocyte mitochondria. These disorders are connected with deficit of enzymes and complexes of electron transfer chain, and can constitute pathogenetic base of the disease. AIM. To study myocardial metabolism in children with cardiomyopathies using positron emission tomography (PET).
Methods: PET studies were carried out in 17 patients (pts.) aged 3-13 years (mean age 7,5-/+3,1) with CMP: hypertrophic (HCMP, n=4), dilated (DCMP, n=9), after radical correction of tetralogy of Fallot (TF, n=4). The dynamic study with [1-11C]-acetate was performed for evaluation of Krebs cycle activity. Rate constant (kmono) and [11C]-activity elimination half-time (T1/2) were calculated using myocardial time-activity curve. Myocardial perfusion was assessed with [13N]-ammonia, glucose metabolism - with [18F]-fluorodeoxyglucose.
Results: Patients with HCMP: left ventricular (LV) perfusion was normal, Krebs cycle activity - decreased; glucose utilization was increased in comparison with normal level in 2, and was normal in 2 other children. Patients with DCMP: LV perfusion was normal; Krebs cycle activity in LV was decreased in 6 and normal - in 3 pts. Overall 2 pts. with CMP had focal perfusion defects. More pronounced decrease of Krebs cycle activity in these regions compared with other LV parts implied development of ischemic injuries. Decrease of glucose utilization in these fields indicated that the injuries were irreversible. Patients with TF: 3 pts. had normal perfusion and Krebs cycle activity; 1 patient with severe hemodynamic disorders had decreased perfusion, glycolysis and Krebs cycle activity in both ventricles indicative of the presence of myocardial dystrophic changes.
Conclusion: Despite normal perfusion children with cardiomyopathy had decreased Krebs cycle activity (mitochondrial dysfunction). Decrease in oxidative metabolism could be compensated by increased glycolysis.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Dynamics of microbial communities and metabolites during the fermentation of Ningxia goji berry wine: An integrated metagenomics and metabolomics approach.
Food Res Int
February 2025
Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China. Electronic address:
Ningxia Goji Berry Wine (NGBW), a traditional Chinese fermented beverage, exhibits complex flavor quality changes during fermentation, the mechanisms of which remain insufficiently elucidated. This study aimed to elucidate the dynamic shifts in physicochemical properties, metabolites, and microbial communities throughout the controlled fermentation process of NGBW. Metabolomic analysis identified 8 key differential volatile metabolites (VOCs) and 406 differential non-volatile metabolites.
View Article and Find Full Text PDF(E)-2-Octenal inhibits Neofusicoccum parvum growth by disrupting mitochondrial energy metabolism and is a potential preservative for postharvest mango.
Food Res Int
February 2025
School of Chemical Engineering, Xiangtan University, Xiangtan 411105, China. Electronic address:
Stem-end rot caused by Neofusicoccum parvum is among the most detrimental diseases affecting postharvest mangoes. The present investigation utilized (E)-2-octenal to manage N. parvum infections, elucidating its mechanism of action.
View Article and Find Full Text PDFAttenuating hyperammonemia preserves protein synthesis and muscle mass via restoration of perturbed metabolic pathways in bile duct-ligated rats.
Metab Brain Dis
January 2025
Hepato-Neuro Laboratory, Centre Hospitalier de l'Université de Montréal (CRCHUM), Université de Montréal, 900, Rue Saint-Denis - Pavillon R, R08.422, Montréal (Québec), H2X 0A9, Canada.
Sarcopenia and hepatic encephalopathy (HE) are complications of chronic liver disease (CLD), which negatively impact clinical outcomes. Hyperammonemia is considered to be the central component in the pathogenesis of HE, however ammonia's toxic effects have also been shown to impinge on extracerebral organs including the muscle. Our aim was to investigate the effect of attenuating hyperammonemia with ornithine phenylacetate (OP) on muscle mass loss and associated molecular mechanisms in rats with CLD.
View Article and Find Full Text PDFLow magnesium (Mg) intake increases the risk of various diseases such as anxiety disorder, depression, and diabetes. However, a reliable biomarker of mild Mg deficiency due to low Mg intake has not yet been identified. We speculate that metabolomics will be effective for biomarker discovery because Mg can affect various metabolic processes in the body.
View Article and Find Full Text PDFDynamics of the mammalian pyruvate dehydrogenase complex revealed by in-situ structural analysis.
Nat Commun
January 2025
Center of Cryo-Electron Microscopy, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
The multi-enzyme pyruvate dehydrogenase complex (PDHc) links glycolysis to the citric acid cycle and plays vital roles in metabolism, energy production, and cellular signaling. Although all components have been individually characterized, the intact PDHc structure remains unclear, hampering our understanding of its composition and dynamical catalytic mechanisms. Here, we report the in-situ architecture of intact mammalian PDHc by cryo-electron tomography.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!