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Evolving a Thermostable Terminal Deoxynucleotidyl Transferase.
ACS Synth Biol
July 2020
Illumina Singapore Pte, Ltd., 29 Woodlands Industrial Park E1, North Tech Building, Singapore 757716.
Terminal deoxynucleotidyl transferase (TdT) catalyzes template free incorporation of arbitrary nucleotides onto single-stranded DNA. Due to this unique feature, TdT is widely used in biotechnology and clinical applications. One particularly tantalizing use is the synthesis of long DNA molecules by TdT-mediated iterative incorporation of a 3' reversibly blocked nucleotide, followed by deblocking.
View Article and Find Full Text PDFRapid Entry into Biologically Relevant α,α-Difluoroalkylphosphonates Bearing Allyl Protection-Deblocking under Ru(II)/(IV)-Catalysis.
Org Lett
December 2019
Department of Chemistry , University of Nebraska, Lincoln , Nebraska 68588-0304 , United States.
A convenient synthetic route to α,α-difluoroalkylphosphonates is described. Structurally diverse aldehydes are condensed with LiFCP(O)(OCHCH═CH). The resultant alcohols are captured as the pentafluorophenyl thionocarbonates and efficiently deoxygenated with HSnBu, BEt, and O, and then smoothly deblocked with CpRu(IV)(π-allyl)quinoline-2-carboxylate (1-2 mol %) in methanol as an allyl cation scavenger.
View Article and Find Full Text PDFSupramolecular Coordination-Directed Reversible Regulation of Protein Activities at Epigenetic DNA Marks.
J Am Chem Soc
November 2018
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education , Wuhan University, Wuhan 430072 , Hubei , China.
In mammals, 5-formylcytosine (5fC) has been identified as an important mark, which plays significant roles in active DNA demethylation and also in epigenetic regulation. It is therefore important to target this epigenetic mark as well as manipulating DNA-protein interactions at this site. A unique feature of 5fC is the presence of a formyl group at the C-5 position.
View Article and Find Full Text PDFα-l-Threose Nucleic Acids as Biocompatible Antisense Oligonucleotides for Suppressing Gene Expression in Living Cells.
ACS Appl Mater Interfaces
March 2018
Department of Chemistry , City University of Hong Kong, Tat Chee Avenue , Kowloon Tong , Hong Kong SAR , China.
Because of the chemical simplicity of α-l-threose nucleic acid (TNA) and its ability to exchange genetic information between itself and RNA, it has attracted significant interest as the RNA ancestor. We herein explore the biological properties and evaluate the potency of sequence-designed TNA polymers to suppress the gene expression in living environments. We found that sequence-specific TNA macromolecules exhibit strong affinity and specificity toward the complementary RNA targets, are highly biocompatible and nontoxic in a living cell system, and readily enter a number of cell lines without using transfecting agents.
View Article and Find Full Text PDFMatrix metalloproteinase-mediation of tumor targeting human recombinant tumor necrosis factor-α fusion protein.
Mol Med Rep
August 2015
Department of Clinical Biochemistry, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
The aim of the present study was to use genetic engineering in order to establish an efficient tumor necrosis factor (TNF)-α fusion protein with low toxicity, which may be used to target tumors. Four types of matrix metalloproteinase (MMP)-mediated tumor targeting human recombinant TNF-α (rhTNF-α) fusion protein vectors were constructed. These were subsequently introduced into Escherichia coli.
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