This study investigates the influence of temperature (T) and osmotic pressure (Pi) on the viability of Escherichia coli K12 during an osmotic treatment. Osmotic shock (dehydration and rehydration within 1 s) in liquid media at different temperatures (4, 10, 30 and 37 degrees C) and different levels of osmotic pressure (26, 30, 35, 40, 82 and 133 MPa) were realized. Results show that a sudden dehydration, below 40 MPa, destroyed up to 80% of the bacterial population for each tested temperature, whereas viability was greater than 90% for an osmotic pressure less than 26 MPa. The influence of T and Pi on the membrane's physical structure is finally considered to explain the results in light of FTIR and electron microscopy study of the influence of temperature and osmotic pressure on E. coli membrane phospholipids conformation.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0005-2736(02)00565-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Widespread occurrence and relevance of phosphate storage in foraminifera.
Nature
January 2025
SUGAR, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka, Japan.
Foraminifera are ubiquitous marine protists that intracellularly accumulate phosphate, an important macronutrient in marine ecosystems and in fertilizer potentially leaked into the ocean. Intracellular phosphate concentrations can be 100-1,000 times higher than in the surrounding water. Here we show that phosphate storage in foraminifera is widespread, from tidal flats to the deep sea.
View Article and Find Full Text PDFEffective repulsive interaction between Janus polymer-grafted nanoparticles adhering to lipid vesicles.
J Chem Phys
January 2025
Department of Physics and Materials Science, The University of Memphis, Memphis, Tennessee 38152, USA.
The adhesion of nanoparticles to lipid vesicles causes curvature deformations to the membrane to an extent determined by the competition between the adhesive interaction and the membrane's elasticity. These deformations can extend over length scales larger than the size of a nanoparticle, leading to an effective membrane-curvature-mediated interaction between nanoparticles. Nanoparticles with uniform surfaces tend to aggregate into unidimensionally close-packed clusters at moderate adhesion strengths and endocytose at high adhesion strengths.
View Article and Find Full Text PDFAcute Osmotic and pH Shock to Bioluminescent Bacteria Is Reversible in Terms of Luminescence Response.
Luminescence
January 2025
Department of Biomedical Science, Acharya Narendra Dev College, University of Delhi, New Delhi, India.
Bioluminescence inhibition (BLI) measurements in bioluminescent bacteria (BB) is perceived as a potential qualitative and quantitative indicator of hazardous materials. Acute but minor fluctuations in osmolarity and pH do not affect the living systems significantly. However, significant BLI is observed from marine BB due to acute osmolarity or pH changes that may affect the bioassay sensitivity.
View Article and Find Full Text PDFPotential Antibacterial of Leaf Sirih Merah Against Enterococcus Faecalis ATCC 29212 Bacteria.
Comb Chem High Throughput Screen
January 2025
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Padjadjaran, Sumedang, Indonesia.
Background: Dental root canal failure is a disease caused by gram-positive bacteria, Enterococcus faecalis. The disease is caused by the bacterial cell wall consisting of a peptidoglycan layer that protects the bacteria from internal osmotic pressure. Peptidoglycan biosynthesis includes many enzymes, such as MurA, Penicillin-binding protein (PBP), and SrtA.
View Article and Find Full Text PDFInducing the Entry of Third Stage Dispersal Juveniles of Bursaphelenchus xylophilus into Cryptobiosis Through Osmotic Regulation.
J Vis Exp
December 2024
1State Key Laboratory of Tree Genetics and Breeding, Institute of Ecological Conservation and Restoration, Chinese Academy of Forestry; Comprehensive Experimental Center in Yellow River Delta of Chinese Academy of Forestry; Tianjin Institute of Forestry Science, Chinese Academy of Forestry;
Cryptobiosis is a state where organisms lose nearly all their internal water and enter anhydrobiosis under extreme environmental stress. The dispersal third-stage juveniles (pre-dauer juveniles, ) of Bursaphelenchus xylophilus can enter cryptobiosis through dehydration and revive upon rehydration when environmental conditions improve. Osmotic regulation is crucial for their survival in this process.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!