AI Article Synopsis
- The study aims to assess how effective preimplantation genetic diagnosis (PGD) with fluorescence in situ hybridization (FISH) is for couples at high risk for genetic diseases.
- Four couples underwent ovarian hyperstimulation, and embryos were biopsied at 6-8 cell stages to perform genetic testing.
- The results showed that out of 12 embryos biopsied, 8 were transferred, leading to one successful pregnancy with a healthy female infant, highlighting PGD's value in preventing genetic disorders.
Article Abstract
Objective: To evaluate the clinical effectiveness of preimplantation genetic diagnosis (PGD) for the couples having a higher risk of genetic diseases with fluorescence in situ hybridization (FISH).
Methods: Ovarian hyperstimulation, oocyte retrieval and in vitro fertilization were performed in 4 cases. Blastomere biopsy were performed from the embryos at the 6 - 8 cell stage. Genetic diagnosis was identified by single blastomere FISH. The female embryos or normal embryos were transferred into uterus.
Results: Twelve embryos were available for embryo biopsy. Twenty cells were collected. Seventeen nuclei were found after fixation. Hybridization signals of 11 embryos were clear and 8 embryos were transferred into uterus. One clinical pregnancy was achieved, and 1 normal female infant was delivered on september 14, 2001.
Conclusion: PGD is very valuable for avoiding selective abortion and birth of affected child of the couples having a higher risk of genetic diseases by detecting embryo sex and chromosome number using FISH.
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