Life in hot environments poses certain constraints on the metabolism of thermophilic organisms. Many universal metabolic intermediates are quite labile compounds, and without protection will rapidly decompose at elevated temperatures. Among these are aminoacyl-tRNAs that are necessarily formed upon functioning of the translation apparatus. Aminoacyl-tRNAs are known to be hydrolyzed rapidly even at moderate temperatures under mild alkaline conditions. We studied the thermal stability of phenylalanyl- and alanyl-tRNA in aqueous solutions in order to evaluate a potential threat posed by high temperatures to these components of the translation machinery of thermophiles. Specific second-order rate constants of the aminoacyl-tRNA hydrolysis reaction were determined in the range 20 degrees -80 degrees C. The activation energy of phenylalanyl- and alanyl-tRNA hydrolysis was found to be about 42 and 23 kJ/mol, respectively. The calculated half-lives of aminoacyl-tRNAs at sub-80 degrees C temperatures vary from several seconds to several dozens of seconds at near-neutral pH. The possible mechanisms counteracting the observed thermolability of aminoacyl-tRNAs in vivo are discussed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00792-002-0285-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Exploring the noncovalent interaction between β-lactoglobulin and flavonoids under nonthermal process: Characterization, physicochemical properties, and potential for lycopene delivering.
Food Chem X
January 2025
Zhejiang Provincial Key Lab for Biological and Chemical Processing Technologies of Farm Product, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, Zhejiang, China.
The poor structure stability and low bioavailability of lycopene (LY) hampers the wide application in food field. Thus, it is crucial to explore novel deliver carrier for LY based on protein-flavonoid complexes. In this study, the noncovalent interaction mechanism between β-lactoglobulin (β-LG) and flavonoids (apigenin (API), luteolin (LUT), myricetin (MY), apigenin-7-O-glucoside, luteolin-7-O-glucoside, and myricetrin) under ultrasound treatment was explored.
View Article and Find Full Text PDFMathematical model and stability of SWCNT- and MWCNT-based nanofluid flow with thermal and chemically reactive effects inside a porous vertical cone.
Front Chem
January 2025
Department of Mathematics and Statistics, Hazara University, Mansehra, Pakistan.
This study investigates the significance of single-walled (SWCNTs) and multi-walled (MWCNTs) carbon nanotubes with a convectional fluid (water) over a vertical cone under the influences of chemical reaction, magnetic field, thermal radiation and saturated porous media. The impact of heat sources is also examined. Based on the flow assumptions, the fundamental flow equations are modeled as partial differential equations (PDEs).
View Article and Find Full Text PDFInsights into the electroactive impact of magnetic nanostructures in PVDF composites small-angle neutron scattering.
Nanoscale
January 2025
Physics Centre of Minho and Porto Universities (CF-UM-UP) and LaPMET - Laboratory of Physics for Materials and Emergent Technologies, University of Minho, 4710-057 Braga, Portugal.
Poly(vinylidene fluoride) (PVDF) is technologically relevant due to its thermal stability; chemical, mechanical and radiation resistance; transparency; biocompatibility; and ease of processing. Several of those applications are related to its high electroactivity, for which the β-phase of the polymer is its most renowned protagonist. In this context, extensive research has been conducted on the crystallization of PVDF in the β-phase, when processed from melt and from solution.
View Article and Find Full Text PDFIn-situ Polymerization Induced Seed-Root Anchoring Structure for Enhancing Stability and Efficiency in Perovskite Solar Modules.
Angew Chem Int Ed Engl
January 2025
Southern University of Science and Technology, Department of Mechanical and Energy Engineering, 1088 Xueyuan Blvd, Nanshan District, 518055, Shenzhen, CHINA.
The escape of organic cations over time from defective perovskite interface leads to non-stoichiometric terminals, significantly affecting the stability of perovskite solar cells (PSCs). How to stabilize the interface composition under environmental stress remains a grand challenge. To address this issue, we utilize thiol-functionalized particles as a "seed" and conduct in situ polymerization of 2,2,3,4,4,4-hexafluorobutyl methacrylate (HFMA) as a "root" at the bottom of the perovskite layer.
View Article and Find Full Text PDFFluoropolymer-Single Crystal Nanocomposite Based Transducer Fabrication for Bio-Imaging.
Adv Healthc Mater
January 2025
Department of Physical Sciences, Indian Institute of Science Education and Research Mohali, Knowledge City, Sector 81, SAS Nagar, Manauli, 140306, India.
Fluoropolymer alone, as an alternative to lead-based piezoelectric materials, has shown multiple challenges to develop useful sensors for solving real-world problems such as photoacoustic, ultrasound pulse echo, and other non-destructive testing. This work demonstrates the fabrication of high frequency and wide bandwidth transducers with fluoropolymer and highly polarizing cubic single crystal Barium titanate (BaTiO) ceramic composite for high resolution in-vivo photo-acoustic and ultrasound imaging. For transducer fabrication, a customized bio-compatible nanocomposite sensor film of PVDF-TrFE (Polyvinylidene fluoride trifluoroethylene)/BaTiO (BTO) is synthesized by drop and dry in heating-cum-electro-poling system for advancing polarization, crystallinity, and higher charge generation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!