Background & Objective: Doc-1R gene is a new gene which was cloned in 1999. Recent studies suggest that Doc-1R gene is a potential tumor suppressor gene. In order to study the function of the Doc-1R gene, The aim of this study was to obtain its genomic sequences and to analyze its expression in the tissues.
Method: According to the cDNA sequences of Doc-1R gene, the gene-specific primers were designed and synthesized. The sequence of Doc-1R gene was cloned through nested PCR using the Genome Walker kit. The sequence and splice donor/acceptor site were analyzed. Using the reverse transcription polymerase chain reaction(RT-PCR), the expression of Doc-1R gene in thirteen tissues, including mouse liver, spleen, pancreas, kidney, lung, intestine, heart, brain, bone, muscle, bladder, ovary, spermary were determined.
Results: The mouse Doc-1R gene has been obtained by two times genomic walking. This gene spans 2,787 bp and contains four exons and three introns. All of the splice donor/acceptor site sequences were in accordance with the consensus "GT-AG" rule. RT-PCR experiments demonstrated Doc-1R gene was expressed in the 13 tissue samples.
Conclusion: The authors have successfully cloned the mouse Doc-1R gene, which will be the foundation for further investigating the function of that gene. The expression pattern suggests that the Doc-1R gene is a housekeeping gene which is important to keep the function of tissues and organs.
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Int J Biol Sci
January 2014
The Research Center for Medical Genomics, MOH Key Laboratory of Cell Biology and Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang 110001, China.
DOC-1R (deleted in oral cancer-1 related) is a novel putative tumor suppressor. This study investigated DOC-1R antitumor activity and the underlying molecular mechanisms. Cell phenotypes were assessed using flow cytometry, BrdU incorporation and CDK2 kinase assays in DOC-1R overexpressing HeLa cells.
View Article and Find Full Text PDFStem Cells Dev
November 2012
School of Dentistry and Dental Research Institute, UCLA, Los Angeles, California 90095, USA.
In this study we present data to support the role for Cdk2ap2 in regulating self-renewal of mouse embryonic stem cells (mESCs) under permissive conditions, and cell survival during differentiation of the mESCs into terminally differentiated cell types. To understand the function of Cdk2ap2 during early development, we generated mESCs with homozygous disruption of the endogenous Cdk2ap2 locus (Cdk2ap2(tr/tr)). The Cdk2ap2(tr/tr) mESCs, when grown in a complete growth medium containing leukemia inhibitory factor (LIF), showed an early differentiation phenotype characterized by flattened colonies and a distinct intercellular boundary.
View Article and Find Full Text PDFJ Clin Endocrinol Metab
December 2005
Section of Endocrinology and Metabolism, University of Illinois at Chicago, 1819 West Polk Street, Chicago, Illinois 60612, USA.
Context: Isolated familial somatotropinoma (IFS) is a rare endocrine disease defined as the occurrence of at least two cases of acromegaly or gigantism in a family that does not exhibit features of Carney complex or multiple endocrine neoplasia type 1. Analysis of the multigenerational expression in families suggests that IFS is inherited as an autosomal dominant disease with incomplete penetrance. The association between the disease and loss of heterozygosity on chromosome 11q13 as well as its linkage to this region has been well established, but the IFS gene still remains unknown.
View Article and Find Full Text PDFBiochem Biophys Res Commun
March 2004
Department of Oral Medicine, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Human DOC-1/CDK2AP1 gene encodes a growth suppressor protein of 12kDa (p12(DOC-1/CDK2AP1)). Recently, p12(DOC-1/CDK2AP1) has been shown to associate with cell cycle proteins including CDK2 and DNA polymerase alpha/primase. It negatively regulates CDK2 activities and suppresses DNA replication.
View Article and Find Full Text PDFDevelopment
November 2003
UMR 7622, CNRS, Université Paris VI, 9 quai Saint Bernard, Bat. C, 75252 Paris, cedex 05, France.
For the success of fertilization, spindles of vertebrate oocytes must remain stable and correctly organized during the arrest in metaphase II of meiosis. Using a two-hybrid screen with MAPK as a bait, we have recently identified MISS (MAPK interacting and spindle stabilizing) which controls mouse oocyte metaphase II spindle stability. Using the same screen, we identify another MAPK partner, DOC1R (Deleted in oral cancer one related), a murine homologue of a potential human tumor suppressor gene.
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