AI Article Synopsis
- The study aimed to create a model of essential fatty acid deficiency (EFAD) in hairless rats to evaluate its suitability for psoriasis research by testing calcipotriol and dithranol.
- The rats were put on a fat-free diet lacking linoleic acid, leading to EFAD symptoms observed within 8 weeks, which included weight loss, skin changes, and altered skin fatty acids.
- Treatments with calcipotriol and dithranol resulted in a significant reduction in epidermal thickness, suggesting that EFAD rats exhibit psoriasis-like symptoms and respond to anti-psoriatic drugs, indicating their potential as a useful research model.
Article Abstract
The objective of this study was to establish essential fatty acid deficiency (EFAD) in hairless rats and investigate the potential of this model as a psoriasis screening model by testing the effects of calcipotriol and dithranol on differentiation and proliferation in the epidermis. Hairless rats were fed with a fat-free diet lacking linoleic acid. The EFAD condition was established within 8 weeks. In order to ensure that this condition had been established, several parameters were measured and observed, i.e. animal weight, water consumption, transepidermal water loss, clinical skin symptoms, histology of the epidermis and fatty acid analysis of serum and skin. Immediately after the EFAD condition had been established, the animals were treated with dithranol ointment or different concentrations of calcipotriol solution. A reduction in epidermal thickness of 15-20% was seen after the treatment with calcipotriol. Dithranol and its coal tar-containing vehicle also showed a reductive effect on epidermal thickness. EFAD hairless rats possess various histological changes resembling psoriasis. These histological changes normalise during treatment with anti-psoriatic drugs as calcipotriol, dithranol and coal tar. The results of the present study indicate that the EFAD rat may be a useful model for studies of anti-psoriatic drugs affecting cell proliferation.
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| http://dx.doi.org/10.1159/000066453 | DOI Listing |
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