AI Article Synopsis
- Biotin synthase only contains [2Fe-2S](2+) clusters when isolated under aerobic conditions.
- Previous studies indicated that under reducing and anaerobic conditions, biotin synthase could be reconstituted with various iron-sulfur clusters including [4Fe-4S](2+) and [2Fe-2S](2+).
- Using (57)Fe Mössbauer spectroscopy, it was found that aerobically grown cells contain a mix of [4Fe-4S](2+) and [2Fe-2S](2+) clusters, supporting the idea that both cluster types may play important roles in the enzyme's function.
Article Abstract
Biotin synthase, the enzyme that catalyzes the last step of the biosynthesis of biotin, contains only [2Fe-2S](2+) clusters when isolated under aerobic conditions. Previous results showed that reconstitution with an excess of FeCl(3) and Na(2)S under reducing and anaerobic conditions leads to either [4Fe-4S](2+), [4Fe-4S](+), or a mixture of [4Fe-4S](2+) and [2Fe-2S](2+) clusters. To determine whether any of these possibilities or other different cluster configuration could correspond to the physiological in vivo state, we have used (57)Fe Mössbauer spectroscopy to investigate the clusters of biotin synthase in whole cells. The results show that, in aerobically grown cells, biotin synthase contains a mixture of [4Fe-4S](2+) and [2Fe-2S](2+) clusters. A mixed [4Fe-4S](2+):[2Fe-2S](2+) cluster form has already been observed under certain in vitro conditions, and it has been proposed that both clusters might each play a significant role in the mechanism of biotin synthase. Their presence in vivo is now another argument in favor of this mixed cluster form.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi026590q | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Radical -Adenosylmethionine Sulfurtransferase MybB Catalyzed Formation of the 4-Thiazolidinone Core in Mycobacidin Represents an Intersection between Primary and Secondary Metabolism.
J Am Chem Soc
January 2025
Department of Chemistry, University of Texas at Austin, Austin, Texas 78712, United States.
Mycobacidin is an antitubercular antibiotic structurally composed of a sulfur-containing 4-thiazolidinone ring, yet its biosynthesis including the mechanism of sulfur incorporation has remained an open question since its discovery in 1952. In this study, the mycobacidin biosynthetic gene cluster is identified from soil-dwelling , and the corresponding biosynthetic pathway starting with 7-oxoheptanoate is characterized. The radical SAM enzyme MybB catalyzes two sulfur insertion reactions, thereby bridging C and C to complete the 4-thiazolidinone heterocycle as the final step in mycobacidin maturation.
View Article and Find Full Text PDFCo-crystal structure of Helicobacter pylori biotin protein ligase with biotinyl-5-ATP.
Acta Crystallogr F Struct Biol Commun
January 2025
Dartmouth Cancer Center, One Medical Center Drive, Lebanon, NH 03756, USA.
Article Synopsis
- Helicobacter pylori is a type 1 carcinogen linked to gastric ulcers and cancer, and research by the Seattle Structural Genomics Center for Infectious Disease focuses on potential treatments targeting this bacterium.
- The study reports on the purification and crystallization of H. pylori biotin protein ligase (HpBPL), an enzyme that plays a crucial role in important metabolic processes and helps H. pylori thrive in the acidic environment of the stomach.
- Despite having low sequence identity with similar proteins, HpBPL shares significant structural similarities with Mycobacterium tuberculosis biotin protein ligase, indicating potential for developing inhibitors that could be effective against HpBPL.
Pathway and protein channel engineering of for improved production of desthiobiotin and biotin.
Synth Syst Biotechnol
November 2024
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
Biotin (vitamin B) is a crucial cofactor for various metabolic processes and has significant applications in pharmaceuticals, cosmetics, and animal feed. , a well-studied Gram-positive bacterium, presents a promising host for biotin production due to its Generally Recognized as Safe (GRAS) status, robust genetic tractability, and capacity for metabolite secretion. This study focuses on the metabolic engineering of .
View Article and Find Full Text PDFBackbone assignments of the biotin carboxyl carrier protein domain of Propionyl CoA carboxylase of Leishmania major and its interaction with its cognate Biotin protein ligase.
Biomol NMR Assign
December 2024
National Institute of Immunology, JNU Campus, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Article Synopsis
- Propionyl CoA carboxylase (PCC) is a multimeric enzyme with α and β subunits that catalyzes the conversion of propionyl CoA to methyl malonyl CoA, with the BCCP domain playing a key role in this process.
- A point mutation in either subunit can disrupt the enzyme's assembly and function, linking PCC to propionic acidemia, a genetic disorder.
- Research on the PCC from Leishmania major (LmPCC) has focused on analyzing its BCCP domain using NMR, revealing important interaction residues and conformational changes related to biotinylation and the enzyme's function.
Comparative Transcriptomic Analysis of Soybean Cyst Nematode Inbred Populations Non-adapted or Adapted on Soybean /-Mediated Resistance.
Phytopathology
October 2024
Department of Plant Pathology and Institute of Plant Breeding, Genetics and Genomics, University of Georgia, Athens, GA 30602.
Soybean cyst nematode (SCN, ) is most effectively managed through planting resistant soybean cultivars, but the repeated use of the same resistance sources has led to a widespread emergence of virulent SCN populations that can overcome soybean resistance. Resistance to SCN HG type 0 (Race 3) in soybean cultivar Forrest is mediated by an epistatic interaction between the soybean resistance genes and . We previously developed two SCN inbred populations by mass-selecting SCN HG type 0 (Race 3) on susceptible and resistant recombinant inbred lines, derived from a cross between Forrest and the SCN-susceptible cultivar Essex, which differ for .
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!