With the advent of recent developments in film manufacturing and experience with photographic filters, the possibility that the traditional x-ray darkroom may no longer be necessary, has been raised. The authors present their experiments using various light filters and film and suggest that with the use of various combinations of lights, films and small tabletop film processors now on the market the darkroom is indeed no longer necessary. Consequently, the elimination of the expense and space for planning darkrooms in new facilities, or replacing them in existing facilities, is a feasible possibility at this time.
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Plant Dis
September 2012
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Geneva, NY 14456.
A DNA macroarray was previously developed to detect major fungal and oomycete pathogens of solanaceous crops. To provide a convenient alternative for researchers with no access to X-ray film-developing facilities, specific CCD cameras or Chemidoc XRS systems, a chromogenic detection method with sensitivity comparable with chemiluminescent detection, has been developed. A fungal (Stemphylium solani) and an oomycete (Phytophthora capsici) pathogen were used to develop the protocol using digoxigenin (DIG)-labeled targets.
View Article and Find Full Text PDFMethods Mol Biol
June 2009
Department of Nutrition and Food Sciences, 101 Poultry Sci Bldg, 260 Lem Morrison Dr., Auburn, AL, 36849, USA.
Western blot detection methods have traditionally used X-ray films to capture chemiluminescence. The increasing costs for film, reagents, and maintenance have driven researchers away from darkrooms to more sensitive and technologically advanced digital imaging systems. Cooled charge coupled devices (CCD) cameras capture both chemiluminescence and fluorescence images, with limitations for each detection method.
View Article and Find Full Text PDFYan Ke Xue Bao
December 1998
Zhongshan Opthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Purpose: To study the influences of moderate- and low intensity of cyclic light exposure on the light damage of rat retina and its relationship with light intensity and exposure duration.
Methods: Twenty five 8-10 weeks old of female Sprague-Dawley rats were divided into 5 groups randomly. One group were as controls reared in dark-room, others were exposured to 12 hour light: 12 hour dark cyclic white xenon light from 3 to 28 days.
Thorax
February 1995
Occupational Lung Disease Unit, Birmingham Heartlands Hospital, UK.
Background: Glutaraldehyde is the best disinfectant for fibreoptic endoscopes. It is also used in the processing of x ray films. A number of studies have reported eye, nose, and respiratory symptoms in exposed workers.
View Article and Find Full Text PDFMultiformat imagers based on laser systems are used for documentation in an increasing number of investigations. The specific problems of quality control are explained and the persistence of film processing in these imager systems of different configuration with (Machine 1: 3M-Laser-Imager-Plus M952 with connected 3M Film-Processor, 3M-Film IRB, X-Rax Chemical Mixer 3M-XPM, 3M-Developer and Fixer) or without (Machine 2: 3M-Laser-Imager-Plus M952 with separate DuPont-Cronex Film-processor, Kodak IR-Film, Kodak Automixer, Kodak-Developer and Fixer) connected film processing unit are investigated. In our checking based on DIN 6868 and ONORM S 5240 we found persistence of film processing in the equipment with directly adapted film processing unit according to DIN and ONORM.
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