AI Article Synopsis
- Flow cytometry is a technique used to analyze cell-surface proteins by producing fluorescence intensity histograms.
- The study explores a new method combining SiZer for smoothing and information theory to assess differences between cytometry histograms.
- Results indicate that this new approach is effective for identifying antibodies based on similar binding patterns on peripheral blood lymphocytes.
Article Abstract
Flow cytometry is a widely available technique for analyzing cell-surface protein expression. Data obtained from flow cytometry is frequently used to produce fluorescence intensity histograms. Comparison of histograms can be useful in the identification of unknown molecules and in the analysis of protein expression. In this study, we examined the combination of a new smoothing technique called SiZer with information theory to measure the difference between cytometry histograms. SiZer provides cross-bandwidth smoothing and allowed analysis in feature space. The new methods were tested on a panel of monoclonal antibodies raised against proteins expressed on peripheral blood lymphocytes and compared with previous methods. The findings suggest that comparing information content of histograms in feature space is effective and efficient for identifying antibodies with similar cell-surface binding patterns.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2244447 | PMC |
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