The aim of the present study was to develop a reliable method for estimating conceptus radiation doses resulting from abdominal radiographic examinations for all trimesters of pregnancy. The method is based on normalized conceptus doses estimated using Monte Carlo modeling. The Monte Carlo N-Particle (MCNP) radiation transport code was employed in the current study. The validity of the MCNP computational approach was verified by comparison with dose data obtained in anthropomorphic phantoms simulating pregnancy at the three trimesters of gestation using thermoluminescence dosimetry (TLD). The results consist of radiation doses normalized to air kerma so that conceptus dose from any technique and x-ray unit used for abdominal radiography can be easily calculated. Normalized conceptus doses are presented for the first, second, and third trimesters of gestation for various kVp and total beam filtration values. Data apply to radiographic systems equipped with high frequency or 3 phase 12 pulse generators. A very good agreement was observed between the normalized conceptus doses estimated by TLD measurements and the MCNP simulation for all periods of gestation (maximum difference 8.1%). The results of MCNP procedures were compared to published data obtained by TLD measurements. Normalized conceptus dose values agree well, with most differences being lower than 10%. The normalized doses obtained in the current study are dependent on field size. However, for small changes in the size of the x-ray field, the change in normalized doses is not considerable. Accurate estimation of conceptus doses due to abdominal conventional x-ray examinations can be made using the dose data provided in the current study.
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Domest Anim Endocrinol
January 2025
Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos, 6627, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address:
This study aimed to characterize histological changes of the maternal-conceptus interface in feto-placental units associated with fetal weight and sex throughout pregnancy. Pregnant Large-White X Landrace gilts(n=18) were euthanized and hysterectomized on gestational days[GDs] 30(n=3), 45(n=5), 60(n=5), and 90(n=5). Intact cross-sections of fetoplacental interface associated with the lightest[LW] and normally-grown[NW] littermates were collected on GD30(n=4 per size).
View Article and Find Full Text PDFProc Natl Acad Sci U S A
November 2024
Department of Biomedical Sciences. University of Missouri, Columbia, MO 65211.
Pregnant women are often prescribed or abuse opioid drugs. The placenta is likely the key to understanding how opioids cause adverse pregnancy outcomes. Maternal oxycodone (OXY) exposure of pregnant mice leads to disturbances in the layer of invasive parietal trophoblast giant cells (pTGC) that forms the interface between the placenta and uterus.
View Article and Find Full Text PDFFASEB J
October 2024
Laboratory of Animal Production Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, Japan.
Embryo survival and pre-implantation development depend on uterine luminal fluid, which is believed to play a role in early embryonic death and infertility in cows. Extracellular vesicles (EVs) in the uterine luminal fluid contain microRNAs (miRNAs), crucial mediators of intercellular communication. miRNAs regulate conceptus-maternal interactions and participate in embryonic development by suppressing gene expression.
View Article and Find Full Text PDFPlacenta
December 2024
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, Grugliasco, 10095, Italy. Electronic address:
Introduction: During the early stage of pregnancy trophoblast cells adapt to adverse uterine environments characterized by oxygen and nutrient deprivation. Autophagy is an intracellular degradation process that aims to promote cell survival in response to stressful conditions. Autophagy activation passes through the mechanistic target of rapamycin (mTOR), also known as a placental nutrient sensor.
View Article and Find Full Text PDFBiol Reprod
October 2024
Division of Animal Sciences, University of Missouri, Columbia, MO, USA.
Conceptus estrogens and prostaglandins have long been considered the primary signals for maternal recognition of pregnancy (MRP) in the pig. However, loss-of-function studies targeting conceptus aromatase genes (CYP19A1 and CYP19A2) and prostaglandin-endoperoxide synthase 2 (PTGS2) indicated that conceptuses can not only signal MRP without estrogens or prostaglandins but can maintain early pregnancy. However, complete loss of estrogen production leads to abortion after day 25 of gestation.
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