Flow cytometric analysis is a technique which allows not only phenotypical characterization of cellular subsets but also allows quantitation of cellular antigens. In this paper we summarize the Molecules of Equivalent Soluble Fluorochromes (MESF) units and the Antibody Binding Capacity (ABC) methods.
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PMN-MDSCs are responsible for immune suppression in anti-PD-1 treated TAP1 defective melanoma.
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Department of General Surgery, Guangzhou Digestive Disease Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, 510013, Guangdong, China.
Introduction: The transporter associated with antigen processing (TAP) is a key component of the classical HLA I antigen presentation pathway. Our previous studies have demonstrated that the downregulation of TAP1 contributes to tumor progression and is associated with an increased presence of myeloid-derived suppressor cells (MDSCs) in the tumor microenvironment. However, it remains unclear whether the elevation of MDSCs leads to immune cell exhaustion in tumors lacking TAP1.
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Department of Histology and Embryology, Faculty of Medicine, Trakya University, Edirne, 22030, Turkey.
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Department of Genetics & Biotechnology, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Youngin, 17104, Republic of Korea.
Abnormal melanin synthesis within melanocytes can result in pigmentary skin disorders. Although pigmentation alterations associated with inflammation are frequently observed, the precise reason for this clinical observation is still unknown. More specifically, although many cytokines are known to be critical for inflammatory skin processes, it is unclear how they affect epidermal melanocyte function.
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