Background: Fertility protection is an urgent clinical problem for prepubertal male oncology patients who undergo either chemotherapy or radiotherapy. As these patients do not have mature sperm to be frozen, there is as yet no effective method to preserve their fertility.
Methods And Results: Single pieces of immature mouse (1.5 x 1.5 x 1.5 mm) or rabbit (2.0 x 2.0 x approximately 3.0 mm) testis were cryopreserved, thawed and transplanted into mouse testes. Histological techniques were used to determine the presence of spermatogenesis, which was restored in both mouse and rabbit testicular pieces, and led to the production of mature sperm after both cryopreservation and syngeneic or xenogeneic transplantation into mouse testes. Using sperm developed in the frozen-thawed transplants, mouse offspring were born after in-vitro microinsemination. Furthermore, rabbit offspring were obtained using rabbit sperm that developed in fresh transplants in a xenogeneic surrogate mouse.
Conclusions: This approach of 'testicular tissue banking' is a promising technique for the preservation of fertility in prepubertal male oncology patients. Xenogeneic transplantation into immunodeficient mice may provide a system for studying spermatogenic failure in infertile men.
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| http://dx.doi.org/10.1093/humrep/17.12.3039 | DOI Listing |
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Article Synopsis
- The study focuses on the cryopreservation of testicular tissue and sperm in bulls, examining how these processes impact sperm quality and cell viability, as well as the expression of the PARP-1 gene.
- Testes were collected from 20 bulls, and sperm was frozen using liquid nitrogen, with testicular tissue treated with cryoprotectants like DMSO and EG before freezing.
- Results showed that post-thaw sperm motility and viability significantly decreased, with DMSO improving cell viability more than EG, and both cryoprotectants influenced gene expression related to DNA repair.
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