AI Article Synopsis
- Ndt80 is a transcriptional activator crucial for meiotic division in the yeast Saccharomyces cerevisiae, especially during the sporulation pathway.
- Ndt80 activates transcription by binding to a specific promoter element known as the MSE, triggering genes essential for meiosis.
- Structural studies have identified a 32 kDa DNA-binding domain of Ndt80 and successfully crystallized it, achieving high-resolution diffraction data.
Article Abstract
The Ndt80 protein is a transcriptional activator that plays a key role in the progression of the meiotic divisions in the yeast Saccharomyces cerevisiae. Ndt80 is strongly induced during the middle stages of the sporulation pathway and binds specifically to a promoter element called the MSE to activate transcription of genes required for the meiotic divisions. Here, the preliminary structural and functional studies to characterize the DNA-binding activity of this protein are reported. Through deletion analysis and limited proteolysis studies of Ndt80, a novel 32 kDa DNA-binding domain that is sufficient for DNA-binding in vitro has been defined. Crystals of the DNA-binding domain of Ndt80 in two distinct lattices have been obtained, for which diffraction data extend to 2.3 A resolution.
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| http://dx.doi.org/10.1107/s0907444902015020 | DOI Listing |
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