The purpose of this study was to find whether the concentration of N-acetylglutamate and ornithine transport into mitochondria would regulate urea synthesis when the dietary protein quality was manipulated. Experiments were done on three groups of rats given diets containing 10 g of gluten, 10 g of casein, or 10 g of whole egg protein/100 g for 10 days. The plasma concentration and urinary excretion of urea, the liver concentration and synthesis of N-acetylglutamate, the liver concentrations of glutamate and lysine, and the liver ornithine transport into mitochondria increased with the decrease in quality of dietary protein. A reverse correlation was observed between the activities of urea cycle enzymes, the plasma concentration of arginine, and urinary excretion of urea under these conditions. N-Acetylglutamate concentration and ornithine transport into mitochondria in the liver were closely correlated with the excretion of urea. These results suggest that greater N-acetylglutamate concentration and ornithine transport into isolated mitochondria in the liver of rats, given the lower quality of protein, stimulate urea synthesis and that the concentrations of glutamate and lysine in the liver are at least partly related to the hepatic N-acetylglutamate synthesis and ornithine transport, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf025635r | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Functional crosstalk of sucrose and G protein signaling in maize thermotolerance by modulating osmoregulation system.
Protoplasma
December 2024
School of Life Sciences, Yunnan Normal University, Kunming, 650092, People's Republic of China.
Sucrose (SUC) is a signaling molecule with multiple physiological functions. G protein is a kind of receptor that converts extracellular first messenger into intracellular second messenger. However, it is little known that SUC interplays with G protein signaling in maize thermotolerance.
View Article and Find Full Text PDFRhizophagus irregularis regulates RiCPSI and RiCARI expression to influence plant drought tolerance.
Plant Physiol
December 2024
State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Arbuscular mycorrhizal fungi (AMF) can transfer inorganic nitrogen (N) from the soil to host plants to cope with drought stress, with arginine synthesis and NH4+ transport being pivotal processes. However, the regulatory mechanism underlying these processes remains unclear. Here, we found that drought stress upregulated expression of genes involved in the N transfer pathway and putrescine and glutathione synthesis in the mycorrhizal structures of Rhizophagus irregularis within alfalfa (Medicago sativa) roots, i.
View Article and Find Full Text PDFHyperornithinemia-Hyperammonemia-Homocitrullinuria Syndrome in Vietnamese Patients.
Medicina (Kaunas)
November 2024
Center of Endocrinology, Metabolism, Genetic/Genomics and Molecular Therapy, Vietnam National Children's Hospital, 18/879 La Thanh, Dong Da, Hanoi 11512, Vietnam.
: Hyperornithinemia-hyperammonemia-homocitrullinuria syndrome (HHH; OMIM 238970) is one of the rare urea cycle disorders. Ornithine carrier 1 deficiency causes HHH syndrome, characterized by failure of mitochondrial ornithine uptake, hyperammonemia, and accumulation of ornithine and lysine in the cytoplasm. The initial presentation and time of diagnosis in HHH highly varies.
View Article and Find Full Text PDFUnveiling the chromate stress response in the marine bacterium Bacillus enclensis AGM_Cr8: a multifaceted investigation.
World J Microbiol Biotechnol
November 2024
School of Biotechnology and Bioinformatics, D.Y. Patil University, Navi Mumbai, India.
Article Synopsis
- - The study introduces Bacillus enclensis AGM_Cr8, a marine bacterium found in polluted Versova Creek, Mumbai, that displays significant tolerance to chromate stress and other heavy metals such as lead and arsenic.
- - Various microscopic techniques show how AGM_Cr8 absorbs chromate, with evidence of both surface interaction and internal bioaccumulation, suggesting a bioreduction mechanism that converts harmful Cr(VI) into less toxic Cr(III).
- - Genome sequencing reveals twenty-two genes related to chromate tolerance and detoxification processes, indicating AGM_Cr8's potential for bioremediation of chromium contamination in the environment.
RNAseq and targeted metabolomics implicate RIC8 in regulation of energy homeostasis, amino acid compartmentation, and asexual development in .
mBio
December 2024
Department of Microbiology and Plant Pathology, University of California, Riverside, California, USA.
Unlabelled: Heterotrimeric G protein signaling pathways control growth and development in eukaryotes. In the multicellular fungus , the guanine nucleotide exchange factor RIC8 regulates heterotrimeric Gα subunits. In this study, we used RNAseq and liquid chromatography-mass spectrometry (LC-MS) to profile the transcriptomes and metabolomes of wild type, the Gα subunit mutants Δ and Δ, and Δ strains.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!