An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium culture. This represents the first molecular attempt to enumerate an uncultured bacterium associated with a mycorrhizal fungus.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC134394 | PMC |
| http://dx.doi.org/10.1128/AEM.68.12.6421-6424.2002 | DOI Listing |
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