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The Transcriptomic and Gene Fusion Landscape of Pleomorphic Salivary Gland Adenomas.
Genes Chromosomes Cancer
January 2025
Sahlgrenska Center for Cancer Research, Department of Laboratory Medicine, University of Gothenburg, Gothenburg, Sweden.
Pleomorphic adenoma (PA) is the most common salivary gland tumor. PAs are characterized by chromosomal rearrangements of 8q12 and 12q14-15, leading to gene fusions involving the PLAG1 and HMGA2 oncogenes. Here, we performed the first comprehensive study of the transcriptomic and gene fusion landscape of 38 cytogenetically characterized PAs.
View Article and Find Full Text PDFPhpC transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Nat Commun
January 2025
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
The assembly of repressive heterochromatin in eukaryotic genomes is crucial for silencing lineage-inappropriate genes and repetitive DNA elements. Paradoxically, transcription of repetitive elements within constitutive heterochromatin domains is required for RNA-based mechanisms, such as the RNAi pathway, to target heterochromatin assembly proteins. However, the mechanism by which heterochromatic repeats are transcribed has been unclear.
View Article and Find Full Text PDFDesigning strong inducible synthetic promoters in yeasts.
Nat Commun
December 2024
Engineering Biology Research Center, Kobe University, Kobe, Japan.
Inducible promoters are essential for precise control of target gene expression in synthetic biological systems. However, engineering eukaryotic promoters is often more challenging than engineering prokaryotic promoters due to their greater mechanistic complexity. In this study, we describe a simple and reliable approach for constructing strongly inducible synthetic promoters with minimum leakiness in yeasts.
View Article and Find Full Text PDFDiscovery of Ureido-Containing Alteropeptilides and Intramolecular Cyclized Alteramides in S16 by Promoter Engineering of Cryptic Biosynthetic Gene Clusters.
J Nat Prod
December 2024
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong 266237, People's Republic of China.
are abundant in the oceans and possess great potential in the synthesis of bioactive natural products. Although many secondary metabolite biosynthetic gene clusters have been identified from genomes, most of their products have not been characterized. In this study, endogenous constitutive promoters with high transcriptional activity were obtained from S16 through RNA-seq and a fluorescence assay of luciferase gene expression.
View Article and Find Full Text PDFA cryptic promoter in the exon of HKR1 drives expression of a truncated form of Hkr1 in Saccharomyces cerevisiae.
PLoS One
November 2024
Food Microbiology Unit, Miyagi University School of Food Industrial Sciences, Sendai, Japan.
Hansenula mrakii killer toxin resistant gene 1 (HKR1) is an intronless, single-exon gene that encodes Hkr1, the signaling mucin of the budding yeast Saccharomyces cerevisiae. HKR1 overexpression confers S. cerevisiae cells with resistance to the HM-1 killer toxin produced by the killer yeast Hansenula mrakii (currently known as Cyberlindnera mrakii).
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