AI Article Synopsis
- The study highlights the use of synthetic pores made from rigid-rod beta barrels for detecting enzymatic reactions through visible fluorescence.
- Improvements in sensitivity by over a thousand times were achieved through simple mutations, showcasing the adaptability of these beta barrels.
- These enzyme activity detectors are valuable for applications in proteomics and environmentally friendly organic synthesis.
Article Abstract
The reversible blockage of synthetic pores formed by rigid-rod beta barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with "naked-eye" fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod beta barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.
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| http://dx.doi.org/10.1126/science.1077353 | DOI Listing |
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