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Longitudinal and Noninvasive Intracellular Recordings of Spontaneous Electrophysiological Activity in Rat Primary Neurons on Planar MEA Electrodes.
Adv Mater
January 2025
Italian Institute of Technology, Genoa, 16163, Italy.
Presently, the in vitro recording of intracellular neuronal signals on microelectrode arrays (MEAs) requires complex 3D nanostructures or invasive and approaches such as electroporation. Here, it is shown that laser poration enables intracellular coupling on planar electrodes without damaging neurons or altering their spontaneous electrophysiological activity, allowing the process to be repeated multiple times on the same cells. This capability distinguishes laser-based neuron poration from more invasive methods like electroporation, which typically serve as endpoint measurement for cells.
View Article and Find Full Text PDFVisualization of primary afferent-evoked excitation of spinal dorsal horn neurons using an intracellular Ca imaging technique in adult rat spinal cord slices.
J Anesth
January 2025
Division of Anesthesiology, Niigata University Graduate School of Medical and Dental Science, 1-757 Asahimachi-dori, Chuo-ku, Niigata, 951-8510, Japan.
Article Synopsis
- This study developed a method for intracellular calcium imaging in adult rat spinal cord slices to understand neuronal activity better.
- The research found that the type of stimulation used (A-fiber vs. C-fiber) affected the calcium responses, with different intensity levels leading to distinct response patterns.
- Results showed that certain drugs, like morphine, significantly suppressed calcium responses, while others, like bicuculline and high doses of tranexamic acid, enhanced them, allowing for insights into pain transmission and modulation in the spinal cord.
An unusual presentation of primary synovial sarcoma of the ethmoid sinus in a 54-year-old woman: A case report and literature review.
Int J Surg Case Rep
January 2025
Department of Pathology, An-Najah National University Hospital, Nablus, Palestine.
Introduction And Importance: Synovial sarcoma is a malignant soft tissue tumor primarily affects the extremities. Its presence in the paranasal sinuses, especially within the ethmoid sinus, is exceedingly rare.
Case Presentation: We report a case of monophasic synovial sarcoma of the ethmoid sinus in a 54-year-old woman, who presented with 3 months history of left eye pain and bulging.
Immunological differences between monophasic and biphasic synovial sarcoma with implications for immunotherapy.
Cancer Immunol Immunother
December 2024
Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands.
Article Synopsis
- Synovial sarcoma is a type of aggressive soft-tissue cancer with limited response to current immunotherapies, highlighting the need for better understanding of the immune cells in its tumor microenvironment.
- Researchers studied 41 patients to analyze the relationship between immune cell types, tumor subtype (biphasic vs. monophasic), disease progression, and response to treatments like chemotherapy.
- Findings indicated that biphasic synovial sarcomas had a higher presence of immune cells, particularly T cells, which correlated with better patient survival, suggesting they may respond more favorably to immunotherapies.
Synovial sarcoma of the larynx, a rare and unusual entity. Case report.
Int J Surg Case Rep
January 2025
Pathology Department, National Medical Center "20 de Noviembre", Mexico City, MX. Mexico.
Introduction And Importance: Synovial sarcoma (SS) is a high-grade malignancy, accounting for 2.5-3.5 % of all primary H&N sarcomas and 0.
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