Endoenzymes associated with haemocyte types in the scallop (Chlamys farreri).

Fish Shellfish Immunol

The Key Laboratory of Mariculture, Ministry of Education, Ocean University of Qingdao, China.

Published: October 2002

Haemocyte types of the scallop (Chlamys farreri) were identified by Giemsa stain and flow cytometry (FCM). Additionally, the activities of peroxidase (POD), phenoloxidase (PO) and alkaline phosphatase (ALP) in haemocytes were analysed by immunocytochemical and biochemical methods. The results indicate that there were two types of haemocytes in the scallop, hyalinocytes and granulocytes, and that POD, PO and ALP were more abundant and more active in granulocytes than in hyalinocytes.

Download full-text PDF

Source
http://dx.doi.org/10.1006/fsim.2001.0402DOI Listing

Publication Analysis

Top Keywords

haemocyte types
8
types scallop
8
scallop chlamys
8
chlamys farreri
8
endoenzymes associated
4
associated haemocyte
4
farreri haemocyte
4
farreri identified
4
identified giemsa
4
giemsa stain
4

Similar Publications

Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes.

Dev Comp Immunol

October 2024

Biology Institute, Universidade Federal Fluminense, Niterói, RJ, 24210-130, Brazil; Department of Biosciences, Swansea University, Singleton Park, Swansea, SA28PP, UK. Electronic address:

Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting.

View Article and Find Full Text PDF
Article Synopsis
  • * A study conducted on 27 P. nobilis individuals from both natural populations and captivity revealed differences in their immune competence, showing that hemolymph from captive animals had a significantly lower Total Hemocyte Count (THC) compared to those from the wild.
  • * Flow cytometry and ultrastructure analysis indicated that captive pen shells exhibited impaired phagocytosis abilities against pathogens, likely caused by PnPV infection disrupting the hemocyte cyt
View Article and Find Full Text PDF

Lake Baikal is one of the largest and oldest freshwater reservoirs on the planet with a huge endemic diversity of amphipods (Amphipoda, Crustacea). These crustaceans have various symbiotic relationships, including the rarely described phenomenon of leech parasitism on amphipods. It is known that leeches feeding on hemolymph of crustacean hosts can influence their physiology, especially under stressful conditions.

View Article and Find Full Text PDF

The bug-killer fly (L.) (Diptera: Tachinidae) forms the respiratory funnel independently of the host's immune response.

Bull Entomol Res

June 2024

Department of Environmental Changes, Faculty of Social and Cultural Studies, Kyushu University, 774 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

In internal parasitism, the respiration strategy within the host's body is as essential as evading attack from the host's immune system. Tachinid flies are parasitoids of terrestrial arthropods, mostly insects, during their larval stage. To obtain oxygen while living in the host body, they build a cylindrical structure known as the respiratory funnel at the aperture opened by the tachinid larva on the host integument or trachea.

View Article and Find Full Text PDF

Harnessing the power of mollusc lectins as immuno-protective biomolecules.

Mol Biol Rep

January 2024

Department of Cardiology, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha Dental College and Hospitals, Saveetha University, Chennai, 600 077, India.

The rapid advancement of molecular research on macromolecules has contributed to the discovery of 'Lectin', a carbohydrate-binding protein which specifically interacts with receptors on the surface of glycans and regulates various cellular activities thereby stimulating immunological functions. Considering the wide variety of sources and immunological significance, research has led to the discovery of lectins in invertebrate molluscs. Such lectins in molluscs mediate active immune response as they lack adaptive immunity.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!