AI Article Synopsis
- Relatively few immune response molecules have been identified in teleost fish compared to mammals, highlighting the need for efficient isolation methods like subtractive hybridization.
- In experiments with Atlantic salmon infected by a virus, cDNA clones resembling mammalian immune genes were discovered, including the CD9 gene.
- The cloned rainbow trout CD9 showed key features of the tetraspanin family and strong expression in various tissues, indicating that these sequences are true teleost CD9 equivalents, prompting further research on their functions through recombinant proteins and antibodies.
Article Abstract
In comparison to mammals, relatively few of the molecules involved in teleost immune responses have been isolated and characterized. A rapid method of isolating molecules important for immune function is subtractive hybridization. One such experiment using infectious hematopoietic necrosis virus-infected Atlantic salmon produced several cDNA clones with similarity to mammalian immune-specific genes, including granzyme M (accession no. AF434669) and CD9. After cloning the rainbow trout version of CD9, sequence analysis showed that both salmonid sequences contained many features of the tetraspanin receptor family to which CD9 belongs. Phylogenetic analysis revealed a close association of the trout and salmon sequences to known CD9 and CD81 receptors. Southern blotting demonstrated that the rainbow trout gene is single copy. Reverse transcriptase PCR showed strong expression of this clone in many tissues, but liver expression was very low - an observation consistent with the clone being a CD9, not a CD81, equivalent. The evidence suggests that the sequences reported here are bona fide teleost CD9 homologues and we are currently producing recombinant proteins and polyclonal antisera for use in functional studies.
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| http://dx.doi.org/10.1007/s00251-002-0506-0 | DOI Listing |
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