AI Article Synopsis
- Researchers used subtractive cloning to discover a specific 1.4 kb mRNA that is widely expressed across different tissues and notably increased in the amygdala of mice treated with morphine.
- This mRNA encodes a protein called 'addicsin' that has similarities to a known protein involved in glutamate transport and shows potential for phosphorylation by PKC.
- The increase in addicsin levels due to morphine may influence glutamate uptake in the amygdala, which could play a role in the development of morphine tolerance and dependence in mice.
Article Abstract
Using subtractive cloning, we identified a 1.4 kb mRNA that was ubiquitously expressed in various tissues; this mRNA was highly up-regulated in amygdala nuclei in mice when morphine was repeatedly administered but not when an opiate-receptor antagonist was co-administered. The mRNA encodes a 23 kDa protein, designated 'addicsin'. This contains two putative PKC-phosphorylation motifs and several hydrophobic regions, and was recovered in a soluble protein fraction of brain lysate. Its primary structure showed 98% identity with that of rat glutamate-transporter-associated protein 3-18 (GTRAP3-18), a putative modulator of neural glutamate-transporter EAAC1. Up-regulation of addicsin expression by morphine may affect glutamate uptake in the amygdala, causing mice to develop morphine tolerance and dependence.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00001756-200211150-00018 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!