The authors describe an epigeal winter seasonal biotope of "culex pipiens autogenicus. The biotope consists of the irrigation system of the large Bervedere park of Tunis city. During the cold and rainy season the irrigation system is abandoned. Many water pools are formed in it in which C. pipiens and Culiseta longiareolata multiply. In summer the operation of the irrigation system brings about the disparition of the nests of larvae. Breeding performed from larvae collected in nature pointed out the autogenous and stenogam characters. Morphologically the specimens correspond to autogenous forms. The importance of the investigations is the evolution of the C. pipiens autogenic species in external nests of larvae during winter was pointed out.
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