Staphylococcus aureus is an important human pathogen, causing a variety of diseases. Major virulence factors of this organism include staphylococcal enterotoxins (SEs) that cause food poisoning and toxic shock syndrome. Our study identified a novel enterotoxin-like protein that is a member of the new subfamily (group V) of pyrogenic toxin superantigens (PTSAgs) and examined its biochemical and immunobiological properties. The gene encoding the SE-like protein is directly 5' of another recently identified PTSAg, SEK. The SE-like protein had a molecular weight of 26000 and an experimentally determined isoelectric point between 7.5 and 8.0. We demonstrated that the PTSAg had many of the biological activities associated with SEs, including superantigenicity, pyrogenicity, and ability to enhance endotoxin shock, but lacked both lethality in rabbits when administered in subcutaneous miniosmotic pumps and emetic activity in monkeys. Recombinant protein stimulated human CD4 and CD8 T cells in a T cell receptor variable region, beta chain (TCRVbeta) specific manner. T cells bearing TCRVbeta 2, 5.1, and 21.3 were significantly stimulated.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bi025977q | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Staphylococcal Enterotoxins: Description and Importance in Food.
Pathogens
August 2024
Department of Food Science and Nutrition, School of Food Engineering, University of Campinas (UNICAMP), Campinas 13083-862, Brazil.
stands out as one of the most virulent pathogens in the genus . This characteristic is due to its ability to produce a wide variety of staphylococcal enterotoxins (SEs) and exotoxins, which in turn can cause staphylococcal food poisoning (SFP), clinical syndromes such as skin infections, inflammation, pneumonia, and sepsis, in addition to being associated with the development of inflammation in the mammary glands of dairy cattle, which results in chronic mastitis and cell necrosis. SEs are small globular proteins that combine superantigenic and emetic activities; they are resistant to heat, low temperatures, and proteolytic enzymes and are tolerant to a wide pH range.
View Article and Find Full Text PDFin Dairy Industry: Enterotoxin Production, Biofilm Formation, and Use of Lactic Acid Bacteria for Its Biocontrol.
Foodborne Pathog Dis
October 2024
InovaLeite - Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, Brazil.
Article Synopsis
- Staphylococcus aureus is a major foodborne pathogen known for producing enterotoxins that cause food poisoning, with classical enterotoxins SEA, SEB, SEC, SED, and SEE responsible for most outbreaks.
- The bacterium thrives on human and animal skin, leading to contamination in food like milk and its products, and it can form biofilms that contribute to persistent food contamination.
- Lactic acid bacteria (LAB) and their bacteriocins are researched for their potential to control S. aureus, but the detailed molecular mechanisms of how they inhibit biofilm formation remain largely unclear and require further study.
Genome-wide high-throughput chromosome conformation capture analysis reveals hierarchical chromatin interactions during early somatic embryogenesis.
Plant Physiol
August 2023
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Somatic embryogenesis (SE), like zygotic embryo development, is a progressive process. Early SE is the beginning of a switch from a somatic to an embryogenic state and is an important stage for initiating chromatin reprogramming of SE. Previous studies suggest that changes in chromatin accessibility occur during early SE, although information on the 3D structure of chromatin is not yet available.
View Article and Find Full Text PDFPrevalence and Virulence Determinants of in Wholesale and Retail Pork in Wuhan, Central China.
Foods
December 2022
Key Laboratory of Preventive Veterinary Medicine in Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
is one of the major foodborne pathogens and can cause serious foodborne illness in humans by foods contaminated with enterotoxins. In recent years, livestock-associated has been a major public health concern for humans and has emerged in various countries globally. China is one of the largest producers of pigs and pork in the world.
View Article and Find Full Text PDFPRP4KA phosphorylates SERRATE for degradation via 20 proteasome to fine-tune miRNA production in .
Sci Adv
March 2022
Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA.
Phosphorylation can quickly switch on/off protein functions. Here, we reported pre-mRNA processing 4 kinase A (PRP4KA), and its paralogs interact with Serrate (SE), a key factor in RNA processing. PRP4KA phosphorylates at least five residues of SE in vitro and in vivo.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!