AI Article Synopsis
- Researchers have developed Methylococcus thermophilus 111p clones that show resistance to tetracycline and examined the stability of this resistance in both the newly created clones and existing Methylomonas rubra 15sh mutants.
- The study found that chromosomal markers for antibiotic and formaldehyde resistance were maintained in both bacterial strains under nonselective conditions, indicating their reliability.
- However, resistance markers encoded by plasmids were not consistently preserved, leading to the conclusion that chromosomal markers are more stable for laboratory and industrial applications.
Article Abstract
A number of Methylococcus thermophilus 111p clones have been obtained which have acquired resistance to tetracycline. The stability of maintenance of marker resistance in these clones and also in already designed Methylomonas rubra 15sh mutants has been investigated. Chromosomal markers resistance to antibiotics or formaldehyde were maintained in the marked strains Methylococcus thermophilus 111p and Methylomonas rubra 15sh after storage in nonselective conditions. The markers of resistance to antibiotics, which were coded by plasmids (pAS8-121 and pULB113), were not always preserved in Methylomonas rubra and Methylococcus thermophilus. The stability of maintenance of chromosomal markers in the investigated methane oxidizing bacteria testifies to the fact that they can be used in laboratory and industrial practice for testing the marked bacteria on selective media. The collection of the marked bacteria-mutants Methylomonas rubra 15sh and Methylococcus thermophilus 111p has been created. These strains stably support the marker resistance to various antibiotics or formaldehyde in unselective conditions.
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Article Synopsis
- Researchers have developed Methylococcus thermophilus 111p clones that show resistance to tetracycline and examined the stability of this resistance in both the newly created clones and existing Methylomonas rubra 15sh mutants.
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