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The precise assessment of emotional states in animals under the combined influence of multiple stimuli remains a challenge in neuroscience research. In this study, multi-dimensional assessments, including high-precision pupil tracking and behavioral analysis, were conducted to investigate the combined effects of fear stimuli and drug manipulation on emotional responses in mice. Mice exposed to foot shocks showed typical freezing and flight behaviors, but neither of these measures could effectively distinguish between dexmedetomidine, isoflurane, and saline groups.

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